Radioimmunoassay

Question 1

Principle of all Immunoassay

Answer 1

which is the recognition of an antigen present in
a sample by antibodies directed against this
antigen

Verry sensitive to vitro technique

Question 2

____________, a doctor of internal medicine,
and Yalow, a nuclear physicist, began
working together in _______ in the
laboratory of Radioisotope Service,
later called the Solomon A. Berson
Research Laboratory at the Bronx
Veterans Administration Hospital in
New York City

Answer 2

Berson, 1950

Question 3

Developed the first radioisotopic technique to studyblood volume and iodine metabolism

Answer 3

Yalow and Berson

Question 4

In _____ Yalow and Berson perfected
their measurement technique and
named it radioimmunoassay (RIA).

Answer 4

1959

Question 5

Types of Assay

Answer 5

Double Antibody RIA - second antibody is added to double-antibody RIA to aid in the
precipitation of the bound main antibody. The unbound labeled medication can be easily
removed after the primary/secondary antibody-antigen complex precipitates.

Coated-tube RIA – The primary antibody is coated on the interior of each tube in coated-
tube RIA. By draining out the supernatant, the unbound labeled medication may be readily
removed.

Question 6

Methods of RIA

Answer 6

Step 1

• We use the target antigen which is labeled radioactively and bound to its specific We will require a limited and known antibody to
  be added in a specific amount in Radioimmunoassay

Step 2

• A sample is then added in order to initiate a reaction competitive in nature, of the labeled antigens from the preparation and the
  unlabeled antigens from the sample with the specific antibodies.

Step 3

• This reaction to the antibodies will release a certain amount of labeled antigen. This
  amount is correlative to the ratio of labeled to unlabeled antigens. A binding curve thus
  obtained allows the amount of antigen in the patient’s serum to be derived

Question 7

Pros of RIA

Answer 7

Extremally sensitive, Highly Specific Test, Process large quantities and Indirect Method

Question 8

Cons of RIA

Answer 8

Hazardous, Requires a Special Storage, High Cost, Long Time, Difficult automation and Long reaction time.

Question 9

Applications of RIA

Answer 9

For drugs, Check Plasma, Vitamins, Anti DNA Antibody and Cancer

Question 10

In Vitro

Answer 10

• Latin for “in glass.”
• Occurs in a controlled environment, such as a test tube or petri dish.
• A straightforward research
  methodology