Quizzes Flashcards

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1
Q

Where should you discard cover glass and slide glass?

A

Sharp’s container

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2
Q

Wild type features of D. melanogaster are?

A

Brick red eyes, tanned body, and straight wings.

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3
Q

What is the role of instagene?

A

it sequesters Magnesium, which is used by DNase’s to degrade DNA.

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4
Q

What is agarose gel electrophoresis?

A

The technology that migrates fragments through the gel from cathode to anode at speeds dependent on their size.

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5
Q

What is the heat shock step of the bacterial transformation supposed to do?

A

Loosen/disrupt the plasma membrane

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6
Q

Why do you run a PCR using primers specific for photosystem II (PSII) chloroplast gene?

A

We use it as a control for the PCR to locate plant sequences.

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7
Q

Do larger or smaller DNA fragments run on agarose gel?

A

Smaller

Larger ones get trapped in pores…

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