Quizzes Flashcards
Where should you discard cover glass and slide glass?
Sharp’s container
Wild type features of D. melanogaster are?
Brick red eyes, tanned body, and straight wings.
What is the role of instagene?
it sequesters Magnesium, which is used by DNase’s to degrade DNA.
What is agarose gel electrophoresis?
The technology that migrates fragments through the gel from cathode to anode at speeds dependent on their size.
What is the heat shock step of the bacterial transformation supposed to do?
Loosen/disrupt the plasma membrane
Why do you run a PCR using primers specific for photosystem II (PSII) chloroplast gene?
We use it as a control for the PCR to locate plant sequences.
Do larger or smaller DNA fragments run on agarose gel?
Smaller
Larger ones get trapped in pores…