Quizlet Flashcards
This discipline includes fundamental research on the biochemistry, physiology, cell biology, ecology, evolution and clinical aspects of microorganisms, including the host response to these agents.
Microbiology
A microscopic organism, which may be single-celled or multicellular.
Microorganism or microbe
It is actually a convex lens of small focal length, which is used for seeing the magnified images of small objects.
Simple microscopes
Used to observe the morphology of organism such as bacteria ,protozoa, fungi, and algae in living (unstained) and nonliving (stained ) state.
Brightfield
Used to detect microorganism in cells ,tissues and clinical specimens
Fluorescence
These ultra-thin sections for electron microscopy are cut on an ultramicrotome with a glass or diamond knife. Glass knives can easily be made in the laboratory and are much cheaper than diamond, but they blunt very quickly and therefore need replacing frequently.
Sectioning
a general term used to describe the process of preserving a sample at a moment in time and to prevent further deterioration so that it appears as close as possible to what it would be like in the living state, although it is now dead.
Fixation
Microorganism that prefer warmer temperature, but can tolerate or endure very cold temperature and can be preserved in the frozen state.
PSYCHRODURIC ORGANISMS
The movement of solvent through a permeable membrane, from a solution having a lower concentration of solute to a solution having a higher concentration of solute.
Osmosis
Are organisms that require sufficient amount of oxygen for growth anfld multiplication
Obligate aerobes
a type of light microscope that uses a compound lens system, meaning, it operates through two sets of lenses to magnify the image of a specimen.
Compound light microscope
a general purpose, nutrient medium used for the cultivation of microbes supporting growth of a wide range of non-fastidious organisms.
Nutrient agar
Is the formulation made after modification which contains an increased level of vancomycin for greater inhibition of gram-positive organisms.
Martin-Lewis Agar
A drug or chemical that inhibits growth and reproduction of microorganism
MICROBISTATIC AGENTS
The use of chemical agent to inhibit the growth of pathogens either temporarily or permanently.
Disinfectant
Catabolization of red blood cells
Hemolysis
a scientific discipline that deals with the application of microorganisms and the knowledge about them.
Applied microbiology
length of a typical humanspermatozoon’s head
5μm –
The hole in the middle of the stage that allows light from the illuminator to reach the specimen.
Aperture
an ultra-thin coating of electrically-conducting material, deposited by low vacuum coating of the sample.
Sputter coating
a device that allows the rotation of an object about a single axis, where an outward force is applied perpendicularly to the axis.
Centrifuge
used to estimate the density of a liquid culture by counting the number of CFU (colony forming units) on an agar or culture plates.
Colony counter
thin metal device with a handle at one end and a looped wire at the other end
Wire inoculating loop
Process that combines dehydration and freezing
LYOPHILIZATION
Refers to the presence of pathogens in blood or tissues
Sepsis
The following are examples of a Pond water protozoa except: Chlamydomonas, Euglena, Vorticella, Paramecium, Bacci, Volvox, Stentor
Bacci
Useful for examining thin spirochetes
Darkfield
produces images by detecting secondary electrons which are emitted from the surface due to excitation by the primary electron beam.
Scanning Electron Microscope (SEM)
A rotating turret that houses the objective lenses. The viewer spins the nosepiece to select different objective lenses.
Nosepiece
Bacteria that partially destroys red blood cells are known as
Alpha hemolytic
These are Non hemolytic, colorless, moist colonies with a characteristic “mousy” odour.
Haemophilus influenzae
________ Also known as broth which are Contained in tubes and are often referred to as “tubed media.”
Liquid media
Used to express the sizes of microorganism
Metric system
Yeasts, Septate hyphae (hyphae with cross-walls), septate hyphae (hyphae without cross-walls) are all examples of what kind of microorganism?
Fungi
A limit to what can be seen using optical instrument
RESOLVING POWER OR RESOLUTION
Specifically inhibits the metabolism and reproduction of bacteria.
BACTERIOSTATIC AGENT
used as a solidifying agent ,much like gelatin is used as a solidifying agent in the kitchen.
Complex polysaccharide
The length of time necessary to sterilize a pure culture at a specified temperature are known as ________
THERMAL Death Time (TDT)
This reduces the number of organism on a surface, it does not penetrates pores and hair follicles to destroy microorganism residing there. It was also applied to the site of surgical incision to destroy local microorganism.
Antiseptic
This is among the uses of a chocolate agar where it permits the differentiation of organism that grow in the medium
Differential medium
This is a very popular liquid medium for use in the bacteriology laboratory as it supports the growth of all categories of bacteria from obligate aerobes to obligate anaerobes
Thioglycollate Broth (THIO)
a chamber that contains appropriate atmosphere and moisture level and set to maintain the appropriate temperature
Incubator
This is done to determine the degree of bacterial contamination in drinking water, milk, and other foods
BACTERIAL POPULATION COUNTS
THE FIRST PHASE OF THE GROWTH, DURING WHICH the bacteria absorb nutrients, synthesize enzymes and prepare for cell division.
Lag phase
The following are FACTORS THAT INFLUENCE THE GROWTH OF MICROORGANISM IN THE BODY, IN THE NATURE, OR IN LABORATORY EXCEPT:
- TEMPERATURE
- pH
- WEATHER
- Moisture content
- available nutrients
- Characteristic of other organism present
Weather
Bacteria that completely destroys red blood cells arw known as _____
Beta hemolytic
It is a selective medium used to cultivate Gram positive organisms.
Phenylethyl alcohol agar (PEA)Phenylethyl alcohol agar (PEA)
Refers to a general purpose, liquid medium that allows the growth of fastidious organisms
Nutrient broth
It is a liquid medium that involves adding a portion of the specimen to the medium
Inoculation
Microbes that actually prefer salty environment
Halophylic
