quiz questions Flashcards
what are antimicrobial peptides
peptides such as Microplusin which have enzymatic activity in order to combat bacteria
name of AMP used in the lab
microplusin
name of vector/ plasmid in which AMP is cloned
pRSET
expression of recombinant AMP will be induced by what molecular compound
IPTG
transformation
transfer of genetic material to cells, such as plasmids
what are competent cells?
competent cells are those that readily accept genetic material/ plasmids
type of E coli cells used to make transformation
E coli BL21
In order to express a gene that is controlled by the T7 promoter, you need to add IPTG to block the function of the lac repressor. T/F?
TRUE
How does electroporation work when transforming bacteria?
electroporation alters the charge on the cell wall, disrupting its permeability and causing it to form pores, so that the DNA can enter
Antimicrobial peptides are cationic or anionic?
cationic
IPTG is a molecular reagent that mimics the function of _______ to induce protein expression.
lactose
name of media to grow E coli cells
LB
T/F? IPTG triggers transcription of the lac repressor to induce recombinant protein expression.
FALSE
name of antibiotic used to grow E coli cells
LB-Amp
define lac operon
a cluster of genes under control of the same promoter, specifically for regulation of lac gene expression
for how long are we going to incubate the E coli cell culture after addition of IPTG?
4 hours
Antimicrobial peptide was isolated from which organism?
Spider A. gomesiana
what does SDS PAGE stand for
sodium dodecyl sulfate polyacrylamide gel electrophoresis
SDS-PAGE is used to separate proteins according to?
size
SDS is a detergent that dissolves _____ molecules.
hydrophobic
after addition of SDS, the protein is denatured and acquires a _____ charge
negative (-)
SDS-PAGE: proteins travel from ____ pole to _____ pole.
negative to positive
what does IMAC stand for?
immobilized metal affinity chromatography
mention one of the metal that has greatest affinity for histidine?
copper/ nickel
how many histidines does our vector have at its tail
6
