quiz pool #6 Flashcards
1) From which organism will we get cells?
Chick embryo.
Where is the airspace in an egg located?
Airspace is found on the blunt end of the egg or simply top portion of the egg
3) How will we keep bacterial contamination to a minimum in our cultures?
a. Using clean tools, and rinsing them in boiling water before and during use
b. Using antibiotics in the culture medium
c. Growing the cells for a short period of time
What is trypsin and what does it do in this experiment?
Trypsin is an proteolytic enzyme that digest the extracellular materials that hold the cells in place, allowing us to isolate the cells so they can be transferred into a culture chamber
5) After treatment with trypsin and pipetting up and down of the culture medium, the solution surrounding the tissue becomes cloudy. What is the cause of the cloudiness? That is, what is being released from the tissue?
The solution is cloudy with single cells released from the tissue
6) a. What are the 2 parts of the flaskettes that we are using? (2 points)
b. To which part will the cells primarily attach? c. What will we do with the cells next week that makes the answer to (b) very useful?
a. it consists of a microscope slide with a plastics vessel attached.
b. microscope slide
c. we will be viewing the cells on the slide with the fluorescent microscope
7) What is a “primary culture”?
Culturing of cells obtained directly from the organism
8) What does “in vitro” mean?
In vitro literally means “in glass”.
9) What does MitoTracker stain?
The mitochondria!!
10) What does formaldehyde do?
Formaldehyde is used to preserve our cultured cells for next week’s lab when they will be examined
11) Are cells still alive after treatment with formaldehyde?
no
