Quiz 3

Question 1

B-glucuronidase

Answer 1

GUS and is used to measure level of transcription from desired promoter

Question 2

Transgene

Answer 2

a gene or genetic material that has been transferred naturallyy

Question 3

Npt

Answer 3

kanamycin/neomycin/gencine resistance gene

Question 4

Hpt

Answer 4

hygromycin phosphotransferase give resistance

Question 5

virD2

Answer 5

makes a nick at a specific site elsewhere on the Ti plasmid and links itself covalently to the 5’ end

Question 6

vir region

Answer 6

mediates the T region located in the Ti plasmid

Question 7

T-DNA

Answer 7

contains genes that code for enzymes synthesizing opines and phytophormones

Question 8

Minimal promoter

Answer 8

minimal promoter should only have a TATA box and nothing else so it wont work without enhancers or silemcers

Question 9

Chimeric protein

Answer 9

protein made of one subunit from transcription from one source and other subunit from forgein subscript

Question 10

Nuclear Targeting Signal

Answer 10

an amino acid sequence that tags a protein for import into the cell nucleus by nuclear transport

Question 11

Transcription Factor

Answer 11

a protein that controls the rate of transcription of genetic info from dna to mRNA

Question 12

Auxin

Answer 12

promotes elongation

Question 13

Cytokinin

Answer 13

promotes cell division

Question 14

Size of an average gene

Answer 14

8kb

Question 15

Size of an average enhance

Answer 15

between 50 and 1500

Question 16

list the 3 essential domains of the bacterial tetR protein

Answer 16

DNA binding domain
tet binding domain
protein-protein interaction domain

Question 17

Can new domains be added anywhere in a protein without disrupting functions

Answer 17

any domain can be put anywhere without losing function but they cannot be inserted into another domain

Question 18

How can we determine whether a particular region of a gene acts as an enhancer or stimulates transcription in some other way? What tests are there?

Answer 18

an enhancer can be moved upstream/downstream/inverted/in an intron from a gene and still work BUT CANNOT WORK IN TRANS
so we would take the region we suspect and clone it in those places, transform, and measure gene expression

Question 19

Why would anyone put a bacterial origin into an artifical transposon designed for use in eukaryotes?

Answer 19

To make cloning back easy- easier to multiply and manipulate genes in e coli

Question 20

Name 1 specific functional motiff found in most eukaryotic promoters

Answer 20

TATAATA box

Question 21

What determines the strength of a promoter

Answer 21

How tight the RNA polymerase binds to the TF

Question 22

What is the function of a polyA tail

Answer 22

responsible for determining where mRNA will be processed to make it functional

Question 23

T or F: in eukaryotes constitutively expressed genes use a single enhancer that is functional in all cells all the time

Answer 23

true - C?GCG?CG motif replaces TATA box and is the constitutively promoter

Question 24

T or F: eukaryotic transcription terminates at defined sequences

Answer 24

False- it is unknown exactly what sequence terminates transcription but it is thought the polyA tail has something to do withit

Question 25

What is the nopaline synthase gene

Answer 25

Gene responsible for producing the carbon compound nopaline which can only be used by agrobacterium

Question 26

What is the octopine synthase gene

Answer 26

in T-DNA ti plasmid expressed in plant host cells

Question 27

what is T-DNA

Answer 27

the specific portion of agrobacterium plasmid that is transferred into a plant and encorporated into the genome

Question 28

can an integrated t-dna move again within the plant

Answer 28

no it lacks a transposase

Question 29

What is the preferred way of introducing a construct from the test tube to a mouse egg

Answer 29

injecting the desired gene into the pronucleus of a fertilized mouse egg

Question 30

in what ways does T DNA transfer resemble plasmid conjugation

Answer 30

a pileus is formed and the DNA moves from agro to plant cells like conjugation (both linear forms)