Quiz 2 Flashcards
Blending Inheritance
Darwin. 2 traits blend in reproduction. Not good because it limits variability.
Gregor Mendal
Pea Plant genetics & punnet squares. Based on alleles passed from parents to offspring.
Particulate Model of Inheritance
Each parent transmits 1 copy Kid receives 2 copies Each copy is an allele Dominant alleles can mask recessive alleles Segregation
Segregation
Copies segregate at the point of reproduction at random.
Alelles
Variants of a gene (P,p)
Genotype
Genetic Makeup of an individual (PP, Pp, pp)
Phenotype
Physical traits of organisms (purple, white)
PP, pp
Homozygote
Pp
Heterozygote
Codominance
Neither allele is masked
DNA
Replicable (Can make copies of itself) Store information Vary by mutation *Hereditary Phosphorus, No sulfer
Miosis
Making of sex cells
Mitosis
Making new body cells
How do you make new cells?
Mitosis or Miosis
Who are the 2 guys that matter to DNA?
Marth Chase & Alfred Hershey
Protien
Sulfur, No phophorus
Rosalind Franklin
Discovered the double helix of DNA through xray crystalorgaphy but some fuck face stole it.
DNA Backbone
Sugar phosphate (carbohydrate) this is the ribbon part
DNA Base
Nitrogenous (AGCT)
A&T
2 bonds
G&C
3 bonds (more stable)
WHAT DO YOU NEED TO KNOW ABOUT THE DNA REPLICATION PROCESS?!?
DNA (transcription) RNA (translation) Amino Acid Protein- Phenotype
Transcription
DNA changed to RNA (U’s not T’s)
Translation
RNA turns into proteins by using three bases to find the correct amino acids
Virus
DNA/RNA in the middle with a protein structure. Reproduces by forcing cells in an organism to make copies of it.
HIV
Targets T Cells in immune system. Poops RNA into T-cells and then they copy and continue out back into the body. Made a primate to human transfer twice.
AZT
First treatment for HIV. Disruption the process of transcription and translation in Tcells
Single Nucleotide Polymorphism
Variety in the populations
Polymerase Chain Reaction
Isolates a small part of the genome
PCR Recipe
Buffer & Water (creates solution)
DNTP’s (ATCG)
Primers (short DNA strands that open the DNA)
DNA Temprate (While sequence of your DNA)
TAQ Polymerase (Enzyme adds nucleotide to DNA strand)
Restriction Enzyme
Cuts somewhere in the DNA sequence depending on alleles.
PCR
Denaturation
Amealing
Extension
Denaturation
Takes a double stranded DNA to a single stranded DNA by splitting through heat (95 degrees)
Amealing
Allows DNA to reform. (Opposite of denaturation) (40-65)
Extension
Adds nucleotides to the strand and repeat this 35 times.
Bacteria Reproduction
Fission (Asexual)
Eukaryotes
Everything except bacteria & arches. Have a true nucleus
• Nucleus contains entire genome
Mitochrodria
Powerhouse of the cell.
Plants
Have buds and roots
have a haploid multicellular stage
Why did plants colonize land?
Sun, nutrients, and fewer enemies.
Plant Colonization benefits
Sunlight
concentrated nutrients
Plants Colonization cons
The land sucks
The land isn’t as wet
Can’t find structure (Need structure from other physical objects or a network of roots.)
Zoom in from Person
Person- made of cells- cells have nucleus- contains the genome- chromosomes- DNA- function of Dan is to code protein.
How do you MAKE DNA?
DNA replication - unzip the double strand
What’s the FUNCTION of DNA?
To make proteins through transcription and translation.
Bacteria
Not mono phyletic because prokaryotes include archea which are more closely related to eucarya than procaria
Virus Tech & GMO Tech
Plasma takes up DNA & bacteria picks up that plasma. Bacteria then make zHella Copies of itself.
The DNA transcript/lates into protein with resistant traits.
Electrophoresis
Increases the permeability
Phylogenetics
Forming and testing hypothesis of evolutionary relationships among species.
Phylogenetic trees
Show evolutionary links
Should be parsimonious
Parsimony
Best tree requires fewer changes
Applications of Phylogenetics
Crime and Law
• Can use to predict flu à make flu shot
• Can use to predict flu à make flu shot
Adaptive radiation
Rapid diversification in novel ecological roles
• Single ancestor diversifies into array of new species
Fire Behavior Triangle
Weather- wind, temperature, humidity, precipitation
Topography- slope or flat
Fuels- fine/heavy, moisture, continuous
PCR Lab Week 1
PRC-DAE
Made millions of copies of the taste region we wanted to isolate
PCR Lab Week 2
Restriction enzyme cut sections for tasters and didn’t cut for non taster alleles
PCR Lab Week 3
Electrophoresis
o Applying electricity to move DNA across a medium
o DNA is negatively charged so it moves from negative to positive
o Large pieces more slow and small move faster
Exotoxins
Bad bacteria 1. proteins secreted by bacteria
Endotoxins
Bad bacteria 2. released when cells die
Animals
- Multicellular heterotrophs (don’t make own food)- feed by ingestion and digestion
- No cell walls
- Have collagen- forms strong fibers in connective tissue and bone
- Most have muscles and nerves
- Diploid dominant
- Usually sexually reproduction
Bloom on Fish
Littler pufferfish is the best fish, stinging catfish is the worst fish
Fish
• Have a head
Vertebrate
• Heart, blood with hemoglobin (to move nutrients), kidneys (to remove waste)
• Paraphyletic
Fish jaws
develop from gill arches- part of gill
Lamprey has no jaw
Candiru- scariest fish
- parasitic small catfish
- attracted to ammonia coming form fish gills- byproduct of breathing
- swim up and implant into gills of other fish
Arthropods
- open circulatory system
- segmented body plan
- 5 walking leg pairs – 4 for swimming
Why are insects diverse?
- Co evolve with plants
- Diverse mouths
- Colonize land
- Evolution of flight
- Internal fertilization
- Complex social systems
- Very old
• Archaea
Type of Bacteria
o Don’t know a lot
o X-treme conditions