Quiz 2 Flashcards
How do you create recombinant DNA?
enzymes and laboratory techniques manipulate and isolate DNA segments
A common technique to separate different fragments of cut DNA so that they can be isolated is ______________________. This technique takes advantage of the negative charge on DNA by using an electrical field to separate DNA molecules based on size.
Electrophoresis
What can occur after finishing electrophoresis? (i.e. what is the final product and what can you do with it)
You can combine different pieces of DNA using DNA ligase. You can insert it anywhere you want.
DNA libraries are collections of recombinant DNA molecules that can be stably maintained and replicated , via ______________, in a suitable host organism.
Cloning Vectors
According to the video PCR, what is the main function of PCR (polymerase chain reaction)?
Provides a way to make more copies of a portion of DNA
According to the video PCR, why do we have to use reverse transcriptase in order to complete PCR to diagnosis the disease COVID-19?
convert the RNA to DNA
______________ mimics the process of DNA replication and an individual reaction is cycled through a series of steps in which each step is analogous to a step in DNA replication.
PCR
During PCR, ______________ is when heat is used to separate strands of double-stranded DNA, _______________ is when primers provide the 3’ OH required for elongation by DNA polymerase, and _____________ is the last step and when the DNA polymerase makes new DNA
Denaturation, Primers, Synthesizing
Who is using DNA fingerprinting?
Law Enforcement
What is DNA fingerprinting?
it takes advantage of short repeating sequences that are going to vary amongst individuals
Which statement does not describe one of the three things we can now do involving gene editing?
Activate Human Genomes, Edit Alleles, and Oncogense
Which statement describes one of the main ways we can edit genes in vivo (we discussed two in class)?
transgenesis
How are using knockin animals (specifically mice) during genetic research useful?
They have a normal allele replaced with an allele that has a specific alteration
A ______________ has a normal allele replaced with an allele that has a specific genetic alteration (i.e. a mutation). Fill in the blank.
mice/ mouse
How are using knockout animals during genetic research useful?
They allow for us to test what different outcomes there are after changing an allele or turning it off
