quiz 2

Question 1

ubiquity of microbes results- BHIA abundance (least to greatest)

Answer 1

sink, floor, phone, hand, toliet, shoe

Question 2

ubiquity of microbes results- BHIA diversity (least to greatest)

Answer 2

phone/hand/sink/toilet, floor, shoe

Question 3

ubiquity of microbes results- SABA ABUNDANCE (l –> g)

Answer 3

sink, hand, floor, toilet, phone, shoe

Question 4

ubiquity of microbes results- SABA DIVERSITY (l –> g)

Answer 4

sink, hand/phone, toilet, floor, shoe

Question 5

which samples were bacteria more abundant than fungi?

Answer 5

floor, toilet, sink, hand

Question 6

which sample had more fungi than bacteria

Answer 6

phone

Question 7

which sample had most bacteria? how many?

Answer 7

shoe, tntc

Question 8

which had the most bacterial diversity? how many?

Answer 8

shoe, 4 different type of colonies

Question 9

which sample had most fungi? how many?

Answer 9

shoe, tntc

Question 10

which had most fungal diversity? how many?

Answer 10

shoe, 14 colonies

Question 11

3 basic bacterial morphologies

Answer 11

bacilli (rods), cocci (spheres), spirilla (spirals)

Question 12

first person to observe microbes using single lens microscope

Answer 12

leeuwenhoek

Question 13

confirmed leewenhook’s observations using compound microscope

Answer 13

robert hooke

Question 14

staphylo-

Answer 14

cluster

Question 15

strepto-

Answer 15

chain of cells

Question 16

coarse focus knob

Answer 16

black, moves nosepiece/slide stage up/down significantly

Question 17

fine focus knob

Answer 17

red, small movements up/down of nosepiece/stage

Question 18

condenser

Answer 18

adjusts opening for the light to come through

Question 19

objective lens

Answer 19

closest to the object, provides initial magnification of image (4x, 10x, 40x, 100x)

Question 20

ocular lens

Answer 20

lens closest to the eye, provides final magnification of image (4x)

Question 21

brightfield illumination

Answer 21

most common type of illumination for ordinary microscopy, object image appears dark against a bright background

Question 22

illumination

Answer 22

the light that passes from the lamp through the object

Question 23

immersion oil

Answer 23

same refractive index as glass, only used on 100x objective lens to keep the rays of light passing from the object to the lens nearly parallel, which allows the max amount of light to enter the lens

Question 24

maginifcation

Answer 24

ratio of apparent size of object when viewed through a lens/ size of the object when viewed with the unaided eye (ratio = power of the lens)

Question 25

parcentered

Answer 25

characteristic of a lens system that keeps object detail centered in the field-of-view as different objective lenses are moved into place

Question 26

parfocal

Answer 26

characteristic of a lens system that keeps an object in focus as different objective lenses are moved into place

Question 27

chain of spheres

Answer 27

streptococci

Question 28

chain of rods

Answer 28

streptobacilli

Question 29

bunch of spheres

Answer 29

staphylococci

Question 30

resolution

Answer 30

clarity/clearness of image b b. b bbbbb bbbbb bb b bbbb

Question 31

culture

Answer 31

collectioon of microbes living on/in media

Question 32

pure culture

Answer 32

all one species of microbe

Question 33

colony

Answer 33

each cell is identical in a pure culture

Question 34

mixed culture

Answer 34

2 or more microbial species

Question 35

aseptic techniques

Answer 35

steps taken to reduce contamination (rocal benches, keeps lids/caps on, flame loop)

Question 36

labeling plates

Answer 36

sec 221 initials microbe name