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Clinical Chemistry > QUIZ > Flashcards

QUIZ Flashcards

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1
Q

IT IS A GLASS OR PLASTIC UTENSILS USED TO TRANSFER LIQUIDS

A

PIPETS

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2
Q

USED FOR DISPENSING LIQUID DURING TITRATION

A

BURETS

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3
Q

USED TO TRANSFER SMALL VOLUMES IN BLOOD GAS ANALYSIS

A

SYRINGES

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4
Q
  • Used for viscous samples
  • Uses mercury as calibrating medium
  • Proper use requires rinsing technique
A

TO CONTAIN (TC)

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5
Q
  • Used for non-viscous samples
  • Uses distilled water as calibrating medium
A

TO DELIVER (TD)

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6
Q

WHAT ARE THE BLOWOUT PIPET

A

SEROLOGIC AND OSTWALD FOLIN

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7
Q

WHAT ARE THE SELF DRAINING PIPET

A

VOLUMETRIC AND MOHR PIPET

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8
Q
  • Graduated uniformly along its length
  • Designed to deliver any amount within its capacity
A

GRADUATED PIPETS

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9
Q

HAS GRADUATION MARKS TO THE TIP AND ITS BLOWOUT

A

SEROLOGIC PIPET

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10
Q

NO GRADUATION MARKS TO THE TIP AND IT IS SELF DRAINING

A

MOHR PIPET

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10
Q

IT DELIVERS AN EXACT VOLUME

A

TRANSFER PIPET

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11
Q

FOR VISCOUS FLUIDS (BLOWOUT)

A

OSTWALD FOLIN PIPET

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12
Q

FOR AQUEOUS SOLUTIONS (SELF DRAINING)

A

VOLUMETRIC PIPET

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13
Q

DELIVERS THE AMOUNT OF < 1ML

A

MICROPIPET

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13
Q

WHAT ARE THE MECHANICAL OR AUTOMATIC PIPET

A
  • MICROPIPET
  • MACROPIPET
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14
Q

Uses hygroscopic substance that take up
water/moisture on exposure to air.

A

DESSICATORS AND DESICCANTS

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14
Q

DELIVERS THE AMOUNT OF > 1ML

A

MACROPIPET

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15
Q
  • Must be level and vibration-free
  • Avoid air currents
  • Kept clean
A

BALANCES

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16
Q
  • For preparation of primary standards
  • Measure exact mass but with lower capacities
  • Operating ranges 0.01 mg to 160 g
A

ANALYTICAL BALANCE

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16
Q

WHAT ARE THE TYPES OF BALANCES

A
  • ELECTRONIC TOP BALANCES
  • ANALYTICAL BALANCE
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17
Q
  • For knowing the mass of substances (greater quantity)
  • Used for preparative experime
A

ELECTRONIC TOP LOADING BALANCE

18
Q

WHAT ARE THE BASIC SEPARATION TECHNIQUES

A
  • CENTRIFUGE
  • FILTRATION
19
Q

A process in which a centrifugal force is used to
separate solid matter from a liquid suspension.

A

CENTRIFUGE

20
Q

The speed/centrifugal force is expresses by?

A
  • RPM - REVOLUTION PER MINUTE
  • RCF - RELATIVE CENTRIFUGAL FORCE
  • GRAVITIES
21
Q

TYPES OF FILTRATIONS USED:

A
  • PAPER
  • POLLY ESTER FIBERS
  • CELLULOSE
  • COLUMN MATERIALS
22
Q
  • Instruments that measure light energy
  • Based on the property of colored solutions to absorb light of specific wavelength.
A

OPTICAL INSTRUMENTS

23
Q
  • Is a form of electromagnetic energy
  • Transmitted via electromagnetic waves
A

LIGHT

24
Q

Measured in nanometer

A

WAVES

25
Q
  • Directly proportional to the amount of light absorbed
  • Inversely proportional to the amt. of transmitted light
A

BEER’S LAW

26
Q

COMPONENTS OF SPECTROPHOTOMETER:

A
  • SAMPLE CELL
  • LIGHT SOURCE
  • ENTRANCE SLIT
  • EXIT SLIT
  • PHOTODETECTOR
  • MONOCHROMATOR
27
Q
  • Measures light emitted by excited atoms
  • Measure sodium and potassium because they are easy to excite.
A

FLAME EMISSION

28
Q

Is used to excite the atoms

A

FLAME USING PROPANE

29
Q

Excited atoms return to the ground state by emmiting?

A

LIGHT ENERGY

30
Q

COMPONENTS OF FLAME EMISSION/FES:

A
  • NEBULIZER
  • PHOTOSENSITIVE DETECTOR
  • BURNER
  • MONOCHROMATOR SYSTEM
31
Q
  • Measures light absorbed by ground state atoms
  • Used to measure concentration of calcium atom
  • 100 times more sensitive than FES
A

ATOMIC ABSORPTION

32
Q

The process of separating the charged constituents
of a sample by means of an electrical current.

A

ELECTROPHORESIS

33
Q

COMPONENTS OF ATOMIC ABSORPTION/AAS:

A
  • LIGHT SOURCE
  • BEAM CHOPPER
  • NEBULIZER
  • BURNER
  • MONOCHROMATOR
  • PHOTODETECTOR
33
Q

MIGRATION OF SMALL ION

A

IONTOPHORESIS

34
Q

MIGRATION OF CHARGED MACROMOLECULES IN A POROUS SUPPORT

A

ZONE ELECTROPHORESIS

35
Q

COMPONENTS OF ELECTROHORESIS:

A
  • POWER SUPPLY
  • BUFFER
  • SUPPORT MEDIUM
  • SAMPLE
  • DETECTING SYSTEM
36
Q

SUPPORT MATERIALS IN ELECTOPHPRESIS:

A
  • CELLULOSE ACCETATE = SEPARATE SERUM PROTEIN INTO 5 BANDS
  • AGAROSE GEL = 10 TO 15 BANDS
  • POLYACRYLAMIDE GEL = > 20 BANDS
37
Q

Separate complex mixtures between mobile and
stationary phase.

A

CHROMATOGRAPHY

38
Q

TYPES OF CHROMATOGRAPHY:

A
  • MOBILE PHASE
  • STATIONARY PHASE
  • COLUMN
  • ELUATE
39
Q

IT CARRIES THE COMPLEX MIXTURE

A

MOBILE PHASE

40
Q

THROUGH WHICH MOBILE PHASE FLOWS

A

STATIONARY PHASE

41
Q

MODES OF SEPARATION IN CHROMATOGRAPHY:

A
  • ABSORPTION
  • PARTITION
  • STERIC EXCLUSION
  • ION EXCHANGE
41
Q

HOLDS THE STATIONARY PHASE

A

COLUMN

42
Q

SEPARATED COMPONENTS

A

ELUATE

43
Q
  • Analytes undergo electrochemical oxidative reductive reactions which can be measured with an electrode-containing instrument
  • Measures current or voltage (potential) generated by the activity of specific ions
A

ELECTRODES

44
Q

ELECTRODE DESIGN:

A
  • REFERENCE ELECTRODE = ELECTRODE WITH A CONSTANT VOLTAGE
  • ANALYRICAL ELECTRODE = MEASURING ELECTRODE

Clinical Chemistry (2 decks)

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