QUIZ Flashcards
IT IS A GLASS OR PLASTIC UTENSILS USED TO TRANSFER LIQUIDS
PIPETS
USED FOR DISPENSING LIQUID DURING TITRATION
BURETS
USED TO TRANSFER SMALL VOLUMES IN BLOOD GAS ANALYSIS
SYRINGES
- Used for viscous samples
- Uses mercury as calibrating medium
- Proper use requires rinsing technique
TO CONTAIN (TC)
- Used for non-viscous samples
- Uses distilled water as calibrating medium
TO DELIVER (TD)
WHAT ARE THE BLOWOUT PIPET
SEROLOGIC AND OSTWALD FOLIN
WHAT ARE THE SELF DRAINING PIPET
VOLUMETRIC AND MOHR PIPET
- Graduated uniformly along its length
- Designed to deliver any amount within its capacity
GRADUATED PIPETS
HAS GRADUATION MARKS TO THE TIP AND ITS BLOWOUT
SEROLOGIC PIPET
NO GRADUATION MARKS TO THE TIP AND IT IS SELF DRAINING
MOHR PIPET
IT DELIVERS AN EXACT VOLUME
TRANSFER PIPET
FOR VISCOUS FLUIDS (BLOWOUT)
OSTWALD FOLIN PIPET
FOR AQUEOUS SOLUTIONS (SELF DRAINING)
VOLUMETRIC PIPET
DELIVERS THE AMOUNT OF < 1ML
MICROPIPET
WHAT ARE THE MECHANICAL OR AUTOMATIC PIPET
- MICROPIPET
- MACROPIPET
Uses hygroscopic substance that take up
water/moisture on exposure to air.
DESSICATORS AND DESICCANTS
DELIVERS THE AMOUNT OF > 1ML
MACROPIPET
- Must be level and vibration-free
- Avoid air currents
- Kept clean
BALANCES
- For preparation of primary standards
- Measure exact mass but with lower capacities
- Operating ranges 0.01 mg to 160 g
ANALYTICAL BALANCE
WHAT ARE THE TYPES OF BALANCES
- ELECTRONIC TOP BALANCES
- ANALYTICAL BALANCE
- For knowing the mass of substances (greater quantity)
- Used for preparative experime
ELECTRONIC TOP LOADING BALANCE
WHAT ARE THE BASIC SEPARATION TECHNIQUES
- CENTRIFUGE
- FILTRATION
A process in which a centrifugal force is used to
separate solid matter from a liquid suspension.
CENTRIFUGE
The speed/centrifugal force is expresses by?
- RPM - REVOLUTION PER MINUTE
- RCF - RELATIVE CENTRIFUGAL FORCE
- GRAVITIES
TYPES OF FILTRATIONS USED:
- PAPER
- POLLY ESTER FIBERS
- CELLULOSE
- COLUMN MATERIALS
- Instruments that measure light energy
- Based on the property of colored solutions to absorb light of specific wavelength.
OPTICAL INSTRUMENTS
- Is a form of electromagnetic energy
- Transmitted via electromagnetic waves
LIGHT
Measured in nanometer
WAVES
- Directly proportional to the amount of light absorbed
- Inversely proportional to the amt. of transmitted light
BEER’S LAW
COMPONENTS OF SPECTROPHOTOMETER:
- SAMPLE CELL
- LIGHT SOURCE
- ENTRANCE SLIT
- EXIT SLIT
- PHOTODETECTOR
- MONOCHROMATOR
- Measures light emitted by excited atoms
- Measure sodium and potassium because they are easy to excite.
FLAME EMISSION
Is used to excite the atoms
FLAME USING PROPANE
Excited atoms return to the ground state by emmiting?
LIGHT ENERGY
COMPONENTS OF FLAME EMISSION/FES:
- NEBULIZER
- PHOTOSENSITIVE DETECTOR
- BURNER
- MONOCHROMATOR SYSTEM
- Measures light absorbed by ground state atoms
- Used to measure concentration of calcium atom
- 100 times more sensitive than FES
ATOMIC ABSORPTION
The process of separating the charged constituents
of a sample by means of an electrical current.
ELECTROPHORESIS
COMPONENTS OF ATOMIC ABSORPTION/AAS:
- LIGHT SOURCE
- BEAM CHOPPER
- NEBULIZER
- BURNER
- MONOCHROMATOR
- PHOTODETECTOR
MIGRATION OF SMALL ION
IONTOPHORESIS
MIGRATION OF CHARGED MACROMOLECULES IN A POROUS SUPPORT
ZONE ELECTROPHORESIS
COMPONENTS OF ELECTROHORESIS:
- POWER SUPPLY
- BUFFER
- SUPPORT MEDIUM
- SAMPLE
- DETECTING SYSTEM
SUPPORT MATERIALS IN ELECTOPHPRESIS:
- CELLULOSE ACCETATE = SEPARATE SERUM PROTEIN INTO 5 BANDS
- AGAROSE GEL = 10 TO 15 BANDS
- POLYACRYLAMIDE GEL = > 20 BANDS
Separate complex mixtures between mobile and
stationary phase.
CHROMATOGRAPHY
TYPES OF CHROMATOGRAPHY:
- MOBILE PHASE
- STATIONARY PHASE
- COLUMN
- ELUATE
IT CARRIES THE COMPLEX MIXTURE
MOBILE PHASE
THROUGH WHICH MOBILE PHASE FLOWS
STATIONARY PHASE
MODES OF SEPARATION IN CHROMATOGRAPHY:
- ABSORPTION
- PARTITION
- STERIC EXCLUSION
- ION EXCHANGE
HOLDS THE STATIONARY PHASE
COLUMN
SEPARATED COMPONENTS
ELUATE
- Analytes undergo electrochemical oxidative reductive reactions which can be measured with an electrode-containing instrument
- Measures current or voltage (potential) generated by the activity of specific ions
ELECTRODES
ELECTRODE DESIGN:
- REFERENCE ELECTRODE = ELECTRODE WITH A CONSTANT VOLTAGE
- ANALYRICAL ELECTRODE = MEASURING ELECTRODE
