quiz Flashcards

1
Q

The symbiotic theory concerning the origin of mitochondria and chloroplasts is supported by:
1.They are found inside eukaryotic cells, just like all bacteria.
2.Many mitochondrial and chloroplast proteins are encoded in the nucleus.
3.They contain ribosomes that are similar in size to prokaryotic ribosomes.
4.They use a genetic code that is completely different than prokaryotes or eukaryotes.

A

They contain ribosomes that are similar in size to prokaryotic ribosomes.

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2
Q

A ribose is a hexamer while a deoxyribose is a pentamer
True
False

A

false

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3
Q

A gene is a sequence of ribonucleotides
Select one:
True
False

A

False

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4
Q

A, C, G, T, & U are found in nucleic acids
Select one:
True
False

A

True

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5
Q

Nucleotides are composed of all the following components EXCEPT.
Select one:
a phosphate group
a five carbon sugar
a base
a double-ring sugar

A

a double-ring sugar

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6
Q

The bond linking adjacent nucleotide building blocks in DNA is called:
Select one:
phosphodiester bond
an ether bond
polynucleotide bond
phosphate bond

A

phosphodiester bond

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7
Q

Nucleotides are joined by linking
Select one:
1.The phosphate on the 3’ end of an incoming nucleotide to the 3’ OH group at the 3’ end of a DNA strand
2.The phosphate on the 5’ end of an incoming nucleotide to the 5’ OH group at the 3’ end of a DNA strand
3.The phosphate on the 3’ end of an incoming nucleotide to the 5’ OH group at the 3’ end of a DNA strand
4.The phosphate on the 5’ end of an incoming nucleotide to the 3’ OH group at the 3’ end of a DNA strand

A

The phosphate on the 5’ end of an incoming nucleotide to the 3’ OH group at the 3’ end of a DNA
strand

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8
Q

Regarding nucleotide sugar structure
Select one:
1.A ribose contains five carbons and so does a deoxyribose
2.A ribose contains six carbons but a deoxyribose contains just five
3.A ribose contains five carbons but a deoxyribose contains six
4.A ribose is a hexamer while a deoxyribose is a pentamer

A

A ribose contains five carbons and so does a deoxyribose

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9
Q

The pyrimidines include:
Select one:
Cytosine and Thymine
Adenine and Guanine
Adenine and Thymine
Adenine and Cytosine

A

Cytosine and Thymine

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10
Q

A nucleotide contains the following components:
Select one:
Sugar, Base, Phosphodiester bond
Sugar, Base, Amino acid
Amino group, Carboxyl Group, Phosphate
Sugar, Phosphate, Base

A

Sugar, Phosphate, Base

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11
Q

The purines include:
Select one:
Adenine and Cytosine.
Adenine and Guanine.
Adenine and Thymine.
Cytosine and Thymine

A

Adenine and Guanine

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12
Q

A lagging strand of DNA is synthesized discontinuously
Select one:
False
True

A

True

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13
Q

SSBs
Select one:
Prevent hydrogen bonding between the sugars of complementary nucleotides
Prevent mRNA strands from binding to gene sequences
Prevent base pairing of complementary sequences on unwound helices
Promote helicase unwinding of double-stranded DNA

A

: Prevent base pairing of complementary sequences on unwound helices

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14
Q

The gaps between Okazaki fragments are
Select one:
Filled in by DNA polymerase III
Filled in by DNA polymerase I
Repaired by DNA polymerase I
Repaired by DNA polymerase III

A

Filled in by DNA polymerase I

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15
Q

In bacterial DNA synthesis the lagging strand is made 5 minutes after the leading strand
Select one:
True
False

A

false

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16
Q

In bacterial DNA replication, most of the DNA is synthesized by the enzyme
Select one:
DNA Taq polymerase
DNA Pol. II
DNA Pol. I
DNA Pol III.

A

pol iii

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17
Q

lacZ, lacy, lacA are structural genes of the
Select one:
lac operon
beta-galactosidase triple gene sequence
lac regulatory region
lac operator site

A

lac operon

18
Q

When a repressor protein binds to the operator sequence
Select one:
Transcription of the downstream gene is up regulated
Transcription of the downstream gene is inhibited
Transcription of the downstream gene is mildly promoted
Transcription of the downstream gene is strongly enhanced

A

Transcription of the downstream gene is inhibited

19
Q

bacterial genomes the minus 10 region refers to a region that is
Select one:
Found in the operator site
Ten nucleotides upstream of a gene
Ten nucleotides downstream from a gene
Is 35 nucleotides downstream of the gene

A

Ten nucleotides upstream of a gene

20
Q

When cAMP levels are high in a cell
Select one:
Glucose levels are low
Lactose levels are low
Lactose levels are high
Glucose levels are high

A

Glucose levels are low

21
Q

A high level of transcription from the Lac operon can be achieved under the following conditions:
Select one:
High glucose, Low cAMP, Low lactose
Low glucose, Low cAMP, Low lactose
High glucose, High cAMP, High lactose
Low glucose, High cAMP, High lactose

A

Low glucose, High cAMP, High lactose

22
Q

Inverted repeats make a:
Select one:
Double stranded loop and a double-stranded stem.
Single-stranded loop and a double-stranded stem
Double-stranded loop and a single-stranded loop
Single-stranded stem and a double-stranded loop

A

Single-stranded loop and a double-stranded stem

23
Q

A sugar cannot act as both a signal molecule and a metabolic substrate in the same transcriptional control mechanism.
Select one:
True
False

A

false

24
Q

Coupled transcription translation is an efficient system that enhances the speed of protein production in eukaryotes.
Select one:
True
False

A

false

25
Q

Shine Dalgarno Sequences
Select one:
Are located along the eukaryotic mRNA and prokaryotic mRNA sequences
Are located along the eukaryotic mRNA
Are located along the 16S ribosomal RNA sequence
Are located along the prokaryotic mRNA

A

Are located along the prokaryotic mRNA

26
Q

Anti codons are found on
Select one:
mRNA
tRNA
rRNA
All three of the above

A

tRNA

27
Q

Amino-acyl tRNA synthetase is an enzyme that
Select one:
Removes amino acids from tRNA and adds them to the growing polypeptide
Attaches amino acids to tRNAs
Reverse transcribes RNA into DNA
Synthesizes non-essential amino acids from essential amino acids

A

Attaches amino acids to tRNAs

28
Q

Finding an 8bp recognition sequence is more likely than finding a 6bp sequence in the same piece of DNA.
True
False

A

false

29
Q

Most restriction endonucleases recognition sequences are:
Select one:
Inverted repeats
Hairpins
Tandem repeats
Palindromes

A

Palindromes

30
Q

The recognition sequence of a restriction endonuclease cannot be found on the bacterium’s own genome
Select one:
True
False

A

false

31
Q

The recognition site for the restriction endonuclease StyI is CCWWGG. The substrate sequences for StyI are therefore:
Select one:
CCTAGG
CCATGG
CCTAGG and CCATGG
None of the above

A

CCTAGG and CCATGG

32
Q

Type I restriction enzymes cleave DNA at random positions, often far removed from the recognition site.
True
False

A

true

33
Q

The enzyme AvaII recognizes the sequence GGTCC. However, it is sensitive to CpG methylation. It will therefore not cut if
methylation occurs in which one of the following sequences:
Select one:
…CGGTCCT…
..AGGTCCC…
…GGGTCCA…
…TGGTCCC

A

CGGTCCT

34
Q

dATPalphaS is a nucleotide that is used in preference to dATP in pyrosequencing. This is because:
Select one:
dATPalphaS is a more stable molecule than dATP

dATP can drive the reaction to light emission even if it is not incorporated into the growing DNA chain

dATP produces a weak light signal when incorporated into the growing chain

dATPalphaS produces a stronger light emission signal than dATP when incorporated into the growing chain

A

The correct answer is: dATP can drive the reaction to light emission even if it is not incorporated into the growing DNA chain

35
Q

Telomeres are bacterial sequences found at the end of bacterial linear DNA
Select one:
True
False

A

false

36
Q

Which of the following is a PCR-based method that makes a cDNA copy of an mRNA.
Select one:
Degenerate PCR
Randomly amplified polymorphic DNA
Inverse PCR
Reverse Transcriptase PCR

A

Reverse Transcriptase PCR

37
Q

Amplicons begin to first appear during the
Select one:
First cycle of PCR
Second cycle of PCR
Third cycle of PCR
Fourth cycle of PCR

A

Third cycle

38
Q

Multiple copies of rRNA genes are found in prokaryotic genomes and eukaryotic genomes. The numbers of these…
Select one:
Are greater in eukaryotes than in prokaryotes
Are roughly six copies in the eukaryotic genome.
Are greater in prokaryotes than in eukaryotes
Are of equivalent numbers in prokaryotes and eukaryotes

A

Are greater in eukaryotes than in prokaryotes

39
Q

If a SNP occurs within a gene, then the gene is described as having more than one:
Select one:
Version
allele.
Variant
Form

A

allele

40
Q

If more than _?_of a population does not carry the same nucleotide at a specific position in the DNA sequence, then this
variation can be classified as a SNP.
Select one:
1%
3%
5%
10%

A

1%

41
Q

Telomeres consist of a tandem repeat of the sequence TTAGGG
Select one:
True
False

A

true

42
Q
A