QUIZ Flashcards

1
Q

You suspect mercury contamination in fish caught from an industrial area. Which statements is true?
a. dry ashing will show the presence of mercury in fish
b. ashing following by metal detection is required for determining presence of mercury
c. HPLC can be used for determining the presence of mercury
d. all the above statement are correct

A

b. ashing following by metal detection is required for determining presence of mercury

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2
Q

The black spot found in crucible after ashing chocolate samples for ash determination.
a. is indicative of incomplete digestion
b. is resulting from the trapping of minerals by carbohydrate in the sample
c. can be removed by reashing after the addition of dilute nitric acid
d. all the above

A

d. all the above

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3
Q

Wet ashing is preferred over dry ashing for which of the following reasons
a. wet ashing allows characterization of ash obtained
b. wet ashing minimize loss of volatile metals/minerals
c. wet ashing is generally faster than dry ashing
d. because of all the above reasons
e. because of the reasons given in B & C

A

e. because of the reasons given in B & C

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4
Q

White fumes during wet ashing carried out on hot plates are suggestive of
a. decomposition of sulfuric acid
b. completion of ashing
c. incomplete digestion

A

a. decomposition of sulfuric acid

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5
Q

The principle agent responsible for digestion of matrix in Plasma ashing is
a. molecular oxygen
b. electro magnetic radiation
c. X-ray
d. Nascent oxygen

A

d. Nascent oxygen

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6
Q

Generally the amount of water insoluble ash will be higher than acid insoluble ash
a. true
b. false

A

a. true

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7
Q

According the FDA proprionic acid is considered as a fatty acid.
true
false

A

false

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8
Q

If you skip the acid hydrolysis part of sample preparation for crude fat determination in meat sample, …
a. the crude fat determination will be an overestimate of actual value
b. the crude fat determined will be an underestimate of actual value
c. it will not affect the crude fat analysis

A

b. the crude fat determined will be an underestimate of actual value

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9
Q

Which of the following ranking is correct for the time required for fat analysis in raw fish ?
a. IR < Mojonnier method < soxhlet
b. IR > Mojonnier method > soxhlet
c. IR < soxhlet < Mojonnier method

A

a. IR < Mojonnier method < soxhlet

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10
Q

Which of the following methods of fat determination are volumetric?
a. soxhlet
b. Gerber
c. Goldfish
d. Mojonnier method
e. Babcock
A, B, C
B and E only

A

B and E only

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11
Q

Why “channeling” does not occur in Soxhlet extraction ?
a. sample in the thimble is always surrounded by solvent
b. the solvent vapour does not pass through sample before condensation
c. both of the above reasons

A

c. both of the above reasons

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12
Q

Goldfish method of fat determination in food is an example of
a. crude fat determination using semi-continuous extraction
b. crude fat determination using continuous extraction
c. crude fat determination using discontinuous extraction

A

b. crude fat determination using continuous extraction

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13
Q

You are tasked with determining protein content in an egg white product. the sample was exposed to boiling temperature that caused coagulation of protein. will it affect the crude protein estimation using Kjeldahl method?
a. yes, because the tertiary structure of protein is lost
b. yes, because protein is degraded
c. no, because the nitrogen content remains the same

A

c. no, because the nitrogen content remains the same

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14
Q

Mushrrom amples showed unusually high crude protein content. what will be the primary reason?
a. Chitin and chitosan content of mushroom
b. copper compounds used as food preservative
c. Salt (NaCl) contetn in the mushroom

A

a. Chitin and chitosan content of mushroom

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15
Q

Which of the following statement best describes Kjeldahl method protein estimation?
a. Kjeldahl method is sensitive and specific
b. Kjeldahl method is sensitive but not specific
c. Kjeldahl method is not senstive but speific

A

b. Kjeldahl method is sensitive but not specific

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16
Q

Potassium sulfate in b. Kjeldahl method is used …
a. to increase the boiling point of H2SO4
b. as catalyst to speed up the reaction
c. to break the metal-ammonia complex

A

a. to increase the boiling point of H2SO4

17
Q

If the glass tube conveying ammonia was not fully immersed in boric acid solution during the distillation of ammonia to boric acid the crude protein estimate will …
a. overestimation
b. underestimation
c. not be affected

A

b. underestimation

18
Q

The major difference between Lowry’s and Biuret method of protein determination
a. Cu1+ and Cu2+ forming coordination complexes with dye molecules and proteins molecules
b. difference in the chemistry of dye binding to proteins
c. oxidation of copper by peptide bonds

A

a. Cu1+ and Cu2+ forming coordination complexes with dye molecules and proteins molecules

19
Q

Specificity of Duma’s method is less than that of Kjeldhal’s method for protein determination because
a. non selective absorption maxima of ammonia and nitrogen
b. Duma’s method will also measure nitrogen form inorganic salts
c. Duma’s method will also measure nitrogen form vitamins

A

b. Duma’s method will also measure nitrogen form inorganic salts

20
Q

Most widely used carrier in Dumas analyzer
chlorine
fluorine
helium
nitrogen

A

helium

21
Q

Silver wool in post combustion tube of Dumas analyzer
a. is optional
b. will absorb halogens and sulfur
c. will absorb CO2
d. will absorb moisture

A

b. will absorb halogens and sulfur

22
Q

Absorbance at 280nm can be used for
a. accurately determining protein content in any liquid food
b. determining nucleic acid content
c. rough estimation of protein content in clear fluids
d. differentiating peptides and protein

A

c. rough estimation of protein content in clear fluids

23
Q

Primary characteristics considered for inclusion of a substance as dietary fiber according to codex alimentarius
a. non-digested by human enzymes
b. below 10 saccharide units
c. should have a beneficial physiological function
d. solely obtained from edible portion of the plant
a, b, c
a & c
a, b, c & d

A

a & c

24
Q

Crude fiber estimation in food is done by
a. acid and alkali treatment of dried defatted sample
b. enzyme treatment of dried defatted sample
c. both are considered crude fiber

A

a. acid and alkali treatment of dried defatted sample

25
Q

(TF) the enzymes used for total dietary fiber analysis are sourced form human small intestine.

A

False

26
Q

Reason for using ethanol in the final step of fibre analysis is
a. to precipitate sugar and amino acids
b. to precipitate soluble fiber
c. to remove minerals

A

b. to precipitate soluble fiber

27
Q

The portion of reside obtained after amyloglucosidase enzyme digestion w/o ethanol precipitation mostly contain
a. soluble dietary fiber
b. insoluble dietary fiber
c. resistant starch

A

b. insoluble dietary fiber

28
Q

The major challenge in estimating total carbohydrate in food is
a. carbohydrate get oxidized fast
b. lack of unique property
c. carbohydrates reacts w/ other food components

A

b. lack of unique property

29
Q

According to codex alimentarius, raffinose is a
polysaccharide
trisaccharide
oligosaccharide
disaccharide

A

trisaccharide

30
Q

Phenol-sulfuric acid methods can be used for the estimation of total carbohydrate in a fruit juice.
a. true, if the sample is prepared to remove interfering compounds
b. false, it only detects reducing sugars
c. true, it can used directly w/o sample preparation

A

a. true, if the sample is prepared to remove interfering compounds

31
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A
32
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A
33
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34
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A
35
Q
A