Quiz 14 - A prescription for Translation

Question 1

DNA is _________ into RNA which is ________ into protien

Answer 1

transcribed; translated

Question 2

According to the central dogma of molecular biology, which of the following is a forbidden direction for the transfer of genetic information?
RNA → DNA
RNA → RNA
DNA → RNA
protein → RNA
choose this answer if all of the above are possible

Answer 2

protein → RNA is forbidden

Question 3

In eukaryotes, RNA pol ____ transcribes mRNA.

Answer 3

RNA Pol II

Question 4

In eukaryotes, RNA pol ____ transcribes tRNA.

Answer 4

RNA Pol III

Question 5

Eukaryotic mRNA is ________cistronic while bacterial mRNA is is ______cistronic

Answer 5

Eukaryotic mRNA = monocistronic

Bacterial mRNA = polycistronic

Question 6

Eukaryotic mRNA and bacterial mRNA are short or long-lived

Answer 6

Eukaryotic mRNA = long lived

Bacterial mRNA = short lived

Question 7

The two types of transcription terminators in Escherichia coli are termed what?

Answer 7

Rho-dependent and Rho-independent

Question 8

Exclusion of intervening sequences are included in ____-________ ________ of eukaryotic mRNA

Answer 8

post-transcriptional modifications

Question 9

RNA editing of eukaryotic mRNAs primarily involves

• adenines
• cytosines
• guanines
• thymines
• uracils

Answer 9

Uracils

RNA editing of eukaryotic mRNAs primarily involves Uracil

Question 10

Which of the following statements about the E. coli RNA polymerase holoenzyme is TRUE?

• it does not require a primer for initiating RNA synthesis
• it is composed of the subunits a2bb’s ( it’s suppose to be alpha beta bate sigma)
• Both A and B
• neither A nor B

Answer 10

Both A and B

Question 11

Consider the following DNA molecule:
5’ TTCCCATTTCGATGGGATACGATGGGCTTATGTGAAAATTTGGGG 3’
3’ AAGGGTAAAGCTACCCTATGCTACCCGAATACACTTTTAAACCCC 5’

What is the sequence of the transcribed mRNA, assuming transcription proceeds left to right?

Answer 11

5’ UUCCCAUUUCGAUGGGAUACGAUGGGCUUAUGUGAAAAUUUGGGG 3’

Question 12

True or False. In any given region of eukaryotic DNA, only one strand can act as the coding strand of a gene

Answer 12

True

Question 13

True or False. Alternative splicing of a single pre-mRNA may yield different mRNA species that code for different polypeptides

Answer 13

True

Question 14

The following DNA fragment is being transcribed by RNA polymerase.
5’ ATTGCC 3’
3’ TAACGG 5’

What is the sequence of transcribed RNA, if the top strand is the noncoding strand?
  5’ AUUGCC 3’
  5’ UAAGCC 3’
  5’ GCCAAU 3’
  5’ CCGUUA 3’

none of the above

Answer 14

None of the above

Question 15

The cap on mRNA consists of a ____________ attached to the ___ end

Answer 15

7-methylguanosine attached to the 5’ end

Question 16

For a given gene, the coding strand of DNA is complementary to ?

Answer 16

The template DNA strand

Question 17

During transcription initiation in bacteria, the promoter is recognized by what?

Answer 17

The sigma factor

Question 18

Reverse transcriptase synthesize _____ that is complementary to an ________

Answer 18

DNA; RNA template

Question 19

Which of the following code for amino acids?
  Intron 
  Ion 
  Exon 
  Exxon 
  Enron

Answer 19

Exon

Question 20

The point of transcription termination is said to be ______ from the promoter.

upstream
down stream

Answer 20

Downstream