Quiz Flashcards

1
Q

Which of the following is
considered an advantage of
molecular diagnostics

A

High throughput

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2
Q

On what year was DNA
discovered

A

1953

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3
Q

Which of the following is an
example of a non-culturable agent

A

Human papilloma virus

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4
Q

Which of the following is an
example of a Target Amplification
Technique

A

PCR

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5
Q

Which of the following is an
example of Probe Amplification
Technique

A

Cycle probe technology

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6
Q

This are short oligonucleotide
sequence derived from the specific
gene or region of interest of the
target pathogen

A

PRIMER

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7
Q

A technique that involves the
detection of DNA target that is
hybridized with an RNA

A

Hybrid capture assay

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8
Q

A technique where unbound
probes are washed and bound
probes are determined by the
presence of label

A

ISH

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9
Q

What year was a highly
throughput sequencer was
produced

A

2005

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10
Q

What is the process of breaking
of hydrogen bonds within
complementary bases at certain
temperature

A

Denaturation

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11
Q

What is added to the organism
DNA for hybridization to occur in
ISH

A

Probe

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12
Q

What method is best used for
detection of HPV DNA

A

Hybrid capture assays

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13
Q

What company developed the
bDNA assay?

A

Chiron

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14
Q

In PCR, what contains the target
region of interest

A

DNA template

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15
Q

What is the cofactor for
polymerase activity

A

Magnesium

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16
Q

What is the temperature of your
annealing cycle

A

50-56

17
Q

What is used to disrupt and
separate DNA in PCR

A

Heat

18
Q

What is added to allow
simultaneous amplification of
target in multiplex PCR

A

Primers

19
Q

What is used to identify or
measure the accumulation of
fluorophore emissions in qPCR

A

Thermocyclers

20
Q

What is the temperature set for
your strand displacement
amplification

A

37-55