Quiz Flashcards
Which of the following is
considered an advantage of
molecular diagnostics
High throughput
On what year was DNA
discovered
1953
Which of the following is an
example of a non-culturable agent
Human papilloma virus
Which of the following is an
example of a Target Amplification
Technique
PCR
Which of the following is an
example of Probe Amplification
Technique
Cycle probe technology
This are short oligonucleotide
sequence derived from the specific
gene or region of interest of the
target pathogen
PRIMER
A technique that involves the
detection of DNA target that is
hybridized with an RNA
Hybrid capture assay
A technique where unbound
probes are washed and bound
probes are determined by the
presence of label
ISH
What year was a highly
throughput sequencer was
produced
2005
What is the process of breaking
of hydrogen bonds within
complementary bases at certain
temperature
Denaturation
What is added to the organism
DNA for hybridization to occur in
ISH
Probe
What method is best used for
detection of HPV DNA
Hybrid capture assays
What company developed the
bDNA assay?
Chiron
In PCR, what contains the target
region of interest
DNA template
What is the cofactor for
polymerase activity
Magnesium
What is the temperature of your
annealing cycle
50-56
What is used to disrupt and
separate DNA in PCR
Heat
What is added to allow
simultaneous amplification of
target in multiplex PCR
Primers
What is used to identify or
measure the accumulation of
fluorophore emissions in qPCR
Thermocyclers
What is the temperature set for
your strand displacement
amplification
37-55
