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Bacte Lab > QUIZ 1 > Flashcards

QUIZ 1 Flashcards

Lab safety

1
Q

True or false

Must be shipped in thick glass or plastic culture bottles

A

true

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2
Q

True or false

Properly sealed with waterproof tape and packed with
absorbent material

A

true

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3
Q

True or false

Labeled and with biohazard symbol

A

true

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4
Q

True or false

Placed in second container (metal tube)

A

true

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5
Q

True or false

Cardboard mailing tube is labeled with etiologic agent
label & biohazard symbol

A

true

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6
Q

Which blood to broth ratio is most useful to for culture?
* a. 1:1
* b: 1:2
* c. 1:10
* d. 10:1

A

c. 1:10

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7
Q

Specimen Transport

for urine colony counts

A

for urine colony counts

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8
Q

True or false

At least two patient identifiers should be used to positively identify a patient prior to specimen collection.

A

true

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9
Q

True or false

Uterine cervical swab in Cary-Blair transport medium is accepted for bacterial
culture.

A

True

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10
Q

True or false

Urine sample stored at 4°C for 24 hours is accepted for bacterial culture.

A

True

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11
Q

Lower respiratory tract specimens include the following, except:
* a. Sputum
* b. Bronchial washings
* c. Bronchoalveolar lavage
* d. Nasopharyngeal swabs

A

d. Nasopharyngeal swabs

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12
Q

used for stool and O&P

A

PVA or Buffer formalin

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13
Q

used for stool culture if its delayed by more than >20hrs

A

Cary-Blair transport medium

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14
Q

holding mediums

A

Stuart’s medium and Amie’s medium

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15
Q

selective agar and CO2 generating tablets

A

JEMBEC System

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16
Q

absorbs fatty acid taht alters the PH changes in culture media

A

Charcoal

17
Q

anticoagulants

most common anti coagulant in microbiology

A

0.025% w/v Sodium polyanethol sulfonate (SPS)

18
Q

anticoagulants

for viral culture and microbacterium spp from blood

A

Heparin

19
Q

Specimen Storage

Refrigerator Temp

A

4°C

20
Q

Specimen Storage

Ambient/Room Temp

A

22°C

21
Q

Specimen Storage

Body Temp

A

37°C

22
Q

Specimen Storage

Freezer Temp

A

-20°C or -70 °C

23
Q

Specimen handling

Superficial
Abscess

A

Container : Aerobic swab with stuart’s or amie’s medium

Patient preparation : wipe the area with 70% alcohol

Instruction : aspirate if possible; Swab along edge of wound

Transport Time : <2 hrs

Storage Before Processing : 24hrs/Room tempreture

24
Q

Specimen handling

Deep Abscess

A

Container : Anaerobic Transporter

Patient preparation : Wipe area with sterile saline or 70% alcohol

Instruction : Aspirate/excise tissue

Transport Time : <2 hrs

Storage Before Processing : 24hrs/Room tempreture

25
Q

Specimen handling

Bone Marrow Aspirate or Blood

A

Container : Blood Culture Media Set
(aerobic/anaerobic/Vacutainer Tube with SPS)

Patient preparation : Disinfect puncture site with 70% alcohol in circular outward motion.
Followed by :
chlorhexidine/iodophor
scrub left for 1 min then
2nd alcohol rinse

Instruction : Draw blood at febrile phase; Draw 2 sets (from right and left arm); Amount depends on manufacturer’s instructions

Transport Time : Within 2 hrs/Room Tempreture

Storage Before Processing : <2 hrs/RT
Incubated at 37C once received and logged in the lab

26
Q

Specimen handling

CSF

A

Container : Sterile, screw cap tube
≥ 1 mL bacteria, virus
≥ 2mL fungi, AFB

Patient preparation : Disinfect with iodine/chlorhexidine before collection

Instruction :

Transport Time : <15 mins/Room tempreture

Storage Before Processing : <24 hrs Incubation at 37C except for viruses (4C up to 3 days)

27
Q

Specimen handling

Urethra

A

Container : Swab moistened with Stuart’s/Amie’s medium, JEMBEC Transport System

Patient preparation : Collect 1 hr after patient’s last urination.

Instruction : Remove exudate from urethral opening

Transport Time : <2hrs/RT

Storage Before Processing : 24 hrs/RT

27
Q

Specimen handling

Rectal Swabs

A

Container : Swab placed in enteric transport medium (Cary-Blair)

Patient preparation :

Instruction : Insert swab ~ 1-1.5 cm past anal sphincter Feces should be visible on swab.

Transport Time : <2hrs/Room tempreture

Storage Before Processing : <24 hrs Room Tempreture

28
Q

Specimen handling

Stool Culture

A

Container : Clean, leakproof containerTransfer feces to enteric transport medium if transport will exceed 60 mins

Patient preparation :

Instruction : For routine culture(Salmonella, Shigella, Campylobacter, etc.)

Transport Time : Within 24 hrs/RT holding time Unpreserved <1hr/RT

Storage Before Processing : 24 hrs 4C <48 hrs RT or 4C

28
Q

Specimen handling

IV, Catheters,
Pins

A

Container : Sterile, screw-cap container
≥ 1 mL bacteria, virus
≥ 2mL fungi, AFB

Patient preparation : Disinfect skin with alcohol before removal

Instruction : IV catheters are cultured quantitatively by rolling the segment back and forth on agar with sterile forceps 4x

Transport Time : <15 mins/Room tempreture

Storage Before Processing : Plate ASAPStore at 4C for <2hrs

29
Q

Specimen handling

Vagina

A

Container : Swab moistened with Stuart’s/Amie’s medium or JEMBEC Transport System

Patient preparation :

Instruction : Remove exudateIf smear is needed, use a 2nd swab

Transport Time : <2hrs/RT

Storage Before Processing : 24 hrs/RT

30
Q

Specimen handling

Prostate

A

Container : Swab moistened with Stuart’s/Amie’s medium
Sterile screw-cap tube

Patient preparation : Clean urethral meatus with soap & water. Massage the prostrate through the rectum.

Instruction : Collect secretions on swab or in tube.

Transport Time : <2 hrs/RT for swab; Immediate if tube/RT

Storage Before Processing : 24 hrs/RT for swab Plate immediately if in tube

31
Q

Specimen handling

BAL, BW

A

Container : BAL, BW

Patient preparation : Sterile, screw-top container

Instruction : Anaerobic culture if sheathed catheter was used

Transport Time : <2hrs/RT

Storage Before Processing : 24 hrs/RT

32
Q

Sputum, Tracheal Suction

A

Container : Sterile, screw-top container

Patient preparation : First Morning Sx;
Instruct Px to brush their
teeth/rinse or gargle with
water prior collection

Instruction : Collect deep cough; Watery sample indicates
presence of saliva
* Expectorated
* Induced Sputum

Transport Time : <2hrs/RT

Storage Before Processing : 24 hrs/RT

33
Q
A
33
Q
A

Bacte Lab (2 decks)

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