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Micr 470L > Quiz 1 > Flashcards

Quiz 1 Flashcards

1
Q

What is physiological saline used for?

A

washing RBCs and diluting serum

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2
Q

What is phosphate buffered saline used for?

A

when pH is important

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3
Q

In an immunological titration, the highest dilution will be the one showing what?

A

agglutination, precipitation, hemagglutination, or color reaction

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4
Q

Define diluate

A

the concentrated substance to be diluted

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5
Q

Define diluent

A

substance making the dilution

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6
Q

What is the formula for the dilution?

A

Diluate: diluate + diluent

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7
Q

What is the dilution factor?

A

reciprocal of the dilution

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8
Q

When preparing a desired volume of diluate, what is the formula to use?

A

desired volume / dilution factor = volume of diluate

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9
Q

What is the formula for determining the amount of diluent needed?

A

amount of diluent * (df - 1)

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10
Q

Why do we use serial dilutions?

A

to make a very concentrated solution very diluted

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11
Q

When you have a certain dilution, but need to make a different one, what is the formula?

A

dilution you want / dilution you have = dilution you need to make

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12
Q

If the dilution is 10% what parts of diluent and diluate would you need?

A

1 part diluate to 9 parts diluent

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13
Q

What are the units of 1 Molar (1M)?

A

1 molecular weight / Liters

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14
Q

How do you convert a dilution to a percentage?

A

divide 100 by df
multiply each side of the dilution by that number

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15
Q

How to find the actually amount you have when given the volume and dilution?

A

multiply the volume by how much you have

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16
Q

What is the formula is you know concentration and volume?

A

C1V1=C2V2

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17
Q

What is a cell density dilution?

A

when the cell density is diluted to achieve a desired cell density

18
Q

How would you solve this problem?
How many mL of diluent would you add to 3mL of diluate to prepare a 1:X dilution?

A

3 mL * diluent(X-1)

19
Q

How would you solve this problem?
How many mL of diluate would you add to 27 mL of diluent to prepare a 1:X dilution?

A

27/(X-1)

20
Q

Why are mice used in the lab?

A

reproduce easily
many genetic strains
docile
cheap

21
Q

What does IP stand for?

A

intraperitoneally

22
Q

What does agglutination mean?

A

clumping resulting from antibodies binding to particular antigen

23
Q

What is used to determine ABO blood type?

A

hemagglutination

24
Q

Will the blood type contain the antigen for the same blood type?

A

yes

25
Q

What are isoantibodies?

A

antibodies produced in one member of a species with specificity for antigens found in another member of the same species

26
Q

Will the blood type contain isoantibodies for the same blood type?

A

no

27
Q

How do you know what the Rh factor is?

A

positive will agglutinate

28
Q

What is hemolytic disease of the newborn?

A

when Rh positive fetal red cells are introduced into an Rh negative mother’s circulation at birth and can destroy Rh positive cells during subsequent pregnancies

29
Q

What does the indirect Coombs test look for?

A

anti-Rh antibodies in mother’s blood

30
Q

What does the direct Coombs test look for?

A

maternal anti Rh antibodies attacking fetal RBC’s

31
Q

What three blood cells have spots?

A

neutrophil, eosinophil, and basophil

32
Q

What two blood cells don’t have spots?

A

lymphocyte and monocyte

33
Q

What type of blood cell has an organish tint?

A

eosinohpil

34
Q

What cells are most dense, and are at the bottom, after cetrifusion, of blood

A

erythorocytes and granulocytes

35
Q

What cells are the least dense, and at the top after cetrifusion, of blood?

A

plasma and plateltes

36
Q

What cells are right under the plasma and platelets after cetrifusion?

A

mononuclear cells

37
Q

What is the formula for the cells/mL?

A

average # cells in one square * 10^4 * df

38
Q

What is the formula for total cell number in tube?

A

(total cells counted/squares counted) * 10^4 * df * volume in tube

39
Q

What is a hemocytometer?

A

dual chamber microscope slide used to enumerate cells in suspension

40
Q

What is the volume of each square in a hemocytometer?

A

10^-4 mL

Micr 470L (1 decks)

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