quick revision weaknesses Flashcards
explain the process of PSE
acute stress
lactic acid build up
rapid acidification
low pH 5.3
= PSE, low water holding capacity and metallic taste, reduction
explain DFD
chronic stress
glycogen depletion
less glycogen to lactic acid
slow acidification
high pH 6.2
= DFD - high water holding capacity, soapy, oxidation
explain cold shortening
rapid chilling to reduce microbial growth, below 10 degrees before rigour mortis. less glycogen to lactic acid, tough meat
how to stop cold shortening
electrical stim within 1 hr - muscle contraction, faster pH fall, depletes ATP, induces early rigour, too much causes PSE
muscle tension decreasing hormones
calpains and cathepsins
inhibits calpins
calpastatin - brahmen cattle = tougher meat
inhibit cathepsins
cystatin
meat flavour
ageing - glutamic and inosinic acid
colour of meat
myoglobin. oxygenation = bright red, mletmyoglobin = brown
pale =
low pH
tenderness
- shear force
- myofibrillar fragmentation index
- taste
boar taint
sex steroids in IM fat, urine odour
water activity
measure of how easy it is for water to become involved in chemical reaction
pschotrophs
pycrophiles
thermophiles
below 7 but optimum is 20-30
15 degrees and below
thermophiles above 45 - 55-65 ideal
D value, Z value
D value: The time required to kill 90% of bacterial population at a certain temp
Z value: Temperature change that is required to change D value by a factor of 10
food spoilage
- chemical change
- freezer burn
- ripening
- stailing
sausage fermentation process
Raw - with fat
Mincing - increase SA
Mixing - salt, nitrate- supresses gram negative flora - growth of streptococci, micrococci, pediococci, lactobacilli, fermented carbs- produce lactic acid - decrease water binding capacity of meat protein
Flora lactobacillus plantarum
Casings - pack
Fermentation - lactic acid bacteria convert ferm carb to lactic acid
Drying and ripening- reduce water content, flavouring compounds produced by breakdown of fat and protein
yogurt + antibiotics
culture not multiplying, pH won’t drop, yogurt won’t set
cheese making
Raw
Pasteurisation
Starter - lactococcus
Incubation - fermentation - lactic acid
Rennant - coagulation
Scalding
Cheddaring - stretch curd
Milling - salting, prevents further starter activity
Moulding
Ripening
Packaging
problems in cheese production
blowing, mould growth, bacteriophages attacking starter
3 class plan/ 2 class plan
2 class - measured by presence or absence test: C- is the presence of organism acceptable and to what level
3 class = same + measured by count or concentration test (M - max level of bacteria per gram)
poultry manure as fertiliser
c.botulism outbreak in sheep and cattle - high mortality
wood smoke
contains formaldehyde, phenol and methanol - antimicrobial activity
how many sample in outbreak investigation
100/target prevalence of pathogen in flock x3
fish pain
nociception, brain anatomy and conscious recognition
shellfish bacteria
vibrio
bacteria causing contamination of normal water and mud
clostridium botulinum - chilling and salting to minimise risk
bacteria found in fish in high water temps
salmonella, contained with water effluents
mycobacteriosis in fish and people
- Fish = Multiple internal granulomas, mortalities, chronic presentation (fish tb)
- Humans = Superficial skin lesions, lethargy - Often seen in people who handle fish = ‘fish handlers disease’