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Histotech Exam 3 > Question Dans Manuel > Flashcards

Question Dans Manuel Flashcards

1
Q

L’hématoxyline de Harris est utilisée sur des coupes de tissus pour colorer quel élément cellulaire?
Graisse
Glycogène
Noyaux
Cytoplasme

A

Noyaux

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2
Q

Comment se nomme le processus de « maturation» de l’hématoxyline?
Hydrolyse
Oxydation
Mordançage
Réductions

A

Oxydations

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3
Q

Comment se nomment les produits chimiques de coloration actifs dans la solution d’hématoxyline
mûre?

Hématéine
Hématine
Hématoxyline
Hémosidérine

A

Hématéine

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4
Q

Quelle est l’étape la plus importante dans la coloration régressive à l’hématoxyline?
Le postmordancage dans l’acide picrique
Utilise station d’hématoxyline contenant de la glycérine
Différenciation dans l’alcool acide
Lavage à l’eau après l’hématoxyline

A

Différenciation dans l’alcool acide

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5
Q

Pendant la coloration à l’hématoxyline et à l’éosine (H&E), quel peut être le résultat si l’ammoniac
n’est pas complètement éliminée par le lavage ?
Décoloration de l’hématoxyline
Mauvaise coloration à l’éosine
Noyaux sous-colorés
Aspect brumeux sur la coloration complétée

A

Mauvaise coloration à Éosine

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6
Q

Laquelle des colorations suivantes nécessite un montage avec un milieu de montage aqueux ?
Fite
Huile rouge O
Grocott
H&E

A

Huile rouge O

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7
Q

Lesquels des éléments suivants peuvent être ajoutés à un milieu de montage aqueux pour
empêcher la migration des colorants d’aniline dans le milieu environnant ?
Surcre de canne
Baume du Canada
Glycérine
Gélatine

A

Sucre de canne

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8
Q

La coloration de Giemsa est plus satisfaisante si le pH se situe entre quelles valeurs?
5.6 et 6,0
6,0 et 6,4
6,4 et 6,9
7,0 et 7,5

A

6,4 et 6,9

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9
Q

Laquelle des solutions d’hématoxyline suivantes doit être utilisée dans les jours qui suivent sa
préparation ?
Harris
Delafield
Gill
Weigert

A

Weigert

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10
Q

Quel est l’organite qui provoque une augmentation de la basophilie dans le cytoplasme?
Réticulum endoplasmique rugueux
Appareil de Golgi
Mitichondrie
Lysosome

A

Réticulum endoplasmique rugueux

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11
Q

La coloration peut être influencée par le fixateur utilisé.
Vrai ou faux

A

Vrai

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12
Q

Le chlorure ferrique est à la fois un mordant et un oxydant.
Vrai ou faux

A

Vrai

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13
Q

L’hématoxyline de Gill est une hématoxyline de fer.
Vrai ou faux

A

Faux

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14
Q

Une augmentation de la température accroît généralement la vitesse de la coloration.
Vrai ou faux

A

Vrai

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15
Q

Lors de la coloration d’un support de lames, on constate que l’eau qui suit les alcools réhydratants devient très trouble. Ceci peut très probablement être corrigé de quelle façon?

Sécher plus complètement les lames suivants
Changer le mode de déparaffinage
Changer les alcools
Changer l’eau

A

Changer les alcools

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16
Q

Les coupes colorées à l’H&E révèlent des marbrures semblables à des pigments bruns et de rares noyaux noir brillant. Ceci est très probablement dû à un milieu de montage qui :
Est dissous dans le xylène
Est devenu trop épais
A été trop dilué
A été appliqué après avoir laissé sécher la diapositive

A

A été appliqué après avoir laissé sécher la diapositive

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17
Q

Les coupes du foie colorées à l’H&E montrent des noyaux très sombres et une certaine coloration bleue du cytoplasme. Ceci est très probablement dû à quoi?
Une quantité importante de Réticulum endoplasmique rugueux
Une coloration excessive avec la solution d’hématoxyline de Meyer
Une différentiation inadéquate de l’hématoxyline
Un séjour trop long dans la solution de bleuissement

A

Une différentiation inadéquate de l’hématoxyline

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18
Q

Les coupes de l’intestin grêle colorées au H&E révèlent des muscles, du collagène et des globules rouges tous colorés de la même nuance de rose. Cela indique quoi?
Que la coloration est correcte
Une mauvaise différentiation de l’éosine
Un bleuissement inadéquat avant l’éosine
Que les lames sont restées trop longtemps dans le xylène

A

Une mauvaise différentiation de l’éosine

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19
Q

Un précipité bleu-noir est visible sur les coupes colorées au H&E. Ceci pourrait probablement être évité à l’avenir en effectuant quoi?
Filter l’hématoxyline
Diminuer la quantité d’acide dans l’hématoxyline
Augmenter le temps de déshydratation avant le blanchiment
Changer la solution de bleuissement plus fréquemment

A

Filter l’hématoxyline

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20
Q

Les sections pour les colorations spéciales ont été accidentellement colorées avec de l’hématoxyline. Pour enlever l’hématoxyline, placez les sections dans quelle solution?
De l’alcool acide
De l’ammoniaque diluée
Du carbonate de lithium
De l’alcool isopropylique

A

De l’alcool acide

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21
Q

Hématéine is formed in Mayer hematoxylin solution by the addition of:
Mercuric oxide
Potassium permanganate
Exposure to air
Sodium iodate

A

Iodate de sodium

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22
Q

Mordants are used to:
Change the refractive index of the tissue
Link tissue constituents more closely to the dye
Help diffentiate stains
Oxidize staining solutions

A

Link tissus more closely to the dye

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23
Q

The combination of a dye and a mordant is called a/an:
Base
Accelerator
Lake
Buffer

A

Lake

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24
Q

Mercuric oxide was used in the original formula for Harris hematoxylin to:
Form hématéine
Prevent oxidation
Serve as the mordant
Stabilize solution

A

Form hématéine

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25
Q

DNA can be demonstrated with:
Éosine
Pyronin
The Feulgen reaction
Fast green

A

The feulgen reaction

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26
Q

All the following are aluminum Hématoxyline EXCEPT:
Gill
Harris
Mayer
Weigert

A

Weigert

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27
Q

Which of the following Hématoxyline is not readily decolorized with acide soltions?
Harris
Delafiled
Weigert
Ehrlich

A

Weigert

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28
Q

Natural resins are rarely used for mounting sections today because they:
Are inherently acidic
Dry too fast
Must be dissolved in toluene
Evaporate from under the coverslip

A

Are inherently acidic

29
Q

Which type of metal salt serves as the mordant in Weigert Hématoxyline
Iron
Aluminium
Mercury
Thungsten

A

Iron

30
Q

Acetic acid is added to Harris Hématoxyline to
Keep heterochromatin from staining
Make nuclear staining more specific
Ripen the hematoxylin
Form a dye lake

A

Make nuclear staining more specific

31
Q

A dye that may be substituted for hematoxylin in routine staining is
Phloxine
Methylene blue
Carmine
Celestine blue

A

Celestine blue

32
Q

Which of the following is a good exemple of polychrome stain
Alcian blue
Congo red
Giemsa
Kinyoun

A

Giemsa

33
Q

The chemical group in dyes that confers the property of color is called a/an
Chromogen
Chromophore
Auxochrome
Mordant

A

Chromophore

34
Q

An auxochrome is a/an
Ionizing group present in dyes
Dye group conferring color
Negatively charged protein
Mordant-dye combination

A

Ionizing group present in dyes

35
Q

Which of the following is formed when hematoxylin is subjected to the action of sodium iodate
A dye lake
Hématéine
Hematin
A polychrome

A

Hématéine

36
Q

Heterochromatin is stained by
Hematoxylin
Éosine
Pyronin
Light green

A

Hématoxyline

37
Q

If placed in a solution with a pH below the isolelectric point, cytoplasmique proteins will be
Basophiles
Acidophiles
Polychromatic
Anionic

A

Acidophiles

38
Q

Differentiating in the H&E stain is an exemple of using
Excess mordant
A weak acid
Oxidizers
Buffers

A

A weak acid

39
Q

A tissu component that takes up a cationioc dye is said to be:
Basophiles
Acidophiles
Chromophoric
Absorbant

A

Basophiles

40
Q

Into which of the following dye categories does Éosine fall
Polychromatic
Cationic
Anionic
Amphoteric

A

Anionic

41
Q

For the best cytoplasmic staining the pH of the Éosine should be between
3,0 and 4,0
4,6 and 5,0
5.6 and 6,5
7.0 and 8,0

A

4,6 and 5,0

42
Q

Éthylène glycol functions in Gill hematoxylin solution to
Oxidize the hematoxylin
Mordant the solution
Increase the acidity
Prevent a surface sheen

A

Increase the acidity

43
Q

Which of the following hematoxylin solution will satin the mucin in goblet cells?
Gill
Mayer
Harris
Weigert

A

Gill

44
Q

Nuclear staining is made more selective by adding which of the following to hematoxylin solutions
Ethyl alcohol
Chloral acid
Ethylene glycol
Acetic acid

A

Acetic acid

45
Q

Hematoxylin is converted to dye lake in the Mayer formula by adding which of the following to the solution
Sodium iodate
Citric acid
Ammonium aluminum sulfate
Chloral hydrate

A

Ammonium aluminum sulfate

46
Q

To link hematoxylin to tissu DNA, which of the following must be added
Acid
Oxidizer
Stabilizer
Mordant

A

Mordant

47
Q

Hematoxylin becomes a dye only when in
Is used progressively
Is oxidized
Influences the isoelectric point of proteins
Is polychromed

A

Is oxidized

48
Q

For the most transparency and clarity when viewing well-stained microscopic sections, the refractive index of the mounting medium should be
Above that of the tissue
Near that of the tissue
Below that of the tissue
Near that of air

A

Near that of the tissue

49
Q

A decrease in section transparency can be caused by using
Xylene as the solvant for the mounting medium
Mounting medium with a refractive Index of 1,51-1,55
Mounting medium that has become too thick
Number 1 coverslips for mouting sections

A

Mounting medium that has become too thick

50
Q

Fixation with a neutral buffered formalin will cause tissue cytoplasm to
Take up more hematoxylin
Take up more eosin
Be more difficult to blue
Resist acid decolonization

A

Take up more hematoxylin

51
Q

If the pH of the staining solution is between 4,6 and 5,0 eosin combines with which of the following tissue chemical groups
-COO-
-NH3+-
-PO3-
N-N

A

-NH3+-

52
Q

True of false
Basic dyes have a negative charge

A

False

53
Q

True of false
Oxidizers are sometimes used for differentiation

A

True

54
Q

True or false
Eosin is differentiated by the dehydrating alcohols

A

True

55
Q

True or false
An increase and decrease in the pH of the staining solution can alter staining by changing tissue and/or dye charges

A

True

56
Q

True or false
Frozen sections can be stained with Toluidine blue O

A

True

57
Q

True or false
Resinous mounting media have an index of refraction much lower than that of the tissue

A

False

58
Q

True or false
Resinous mounting media are usually dissolved in toluene, xylene or xylene substitutes

A

True

59
Q

True or false
The more the better is a good practice for applying mounting resin

A

False

60
Q

True or false
The nucleolus of plasma cells is stained green with methyl Green-Pyronin technique

A

False

61
Q

True or false
Bouin solution is a good fixative for tissue to be stained with the Feulgen reaction

A

False

62
Q

Microscopic examination of an H&E stained sections shows nuclei with well-defined chromatin patterns, crisp nuclear membranes, and very pale pink staining of the cytoplasm and erythrocytes. These results indicate:
Over differentiation with acid alcohol
The use of over ripened hematoxylin
A probalbloe pH problem with the eosin
The propret staining of an H&E

A

A probalbloe pH problem with the eosin

63
Q

An H&E stained section shows reddish brown stained nuclei, pink cytoplasm, and bright rose-red erythrocytes. These results indicate:
Prolonged dehydration in lower alcohols
The use of over ripened hematoxylin
A probable pH problem with the eosin
Overtreatement with ammonium hydroxide

A

The use of over ripened hematoxylin

64
Q

H&E stained sections show very uneven staining, with some areas of the slide well-stained and the other areas unstained. These results indicate that most likely the:
Staining solutions were too dilute
Sections were cut too thin
Paraffin was not completely removed
Sections were over fixed

A

Paraffine was not completely removed Sections

65
Q

Water bubbles are seen microscopically on H&E stained sections. This could probably be prevented in the future by:
Drying the slides completely before beginning the stain
Changing the hydrating alcohols more frequently
Making sure that the dehydration steps is complete
Increasing the time in the last xylene step before coverslipping

A

Making sure that the dehydration steps is complete

66
Q

Microscopic examination of an H&E stained sections of kidney is very difficult. Some areas of the tissue cannot be brought into focus, while other areas show excellent detail. This is most likely due to
Incomplete dehydration before mounting
Mounting medium on top of the glass cover
Air bubbles trapped during flotation
Too much heat during processing

A

Mounting medium on top of the glass cover

67
Q

H&E staining sections show hazy blue nuclei, but recuts from tissue processed 1 week previously and stained in the same basket show excellent nuclear staining. One possible cause of the problem is:
The use of too much heat during processing
Incomplete dehydration before mounting
The use of overripened hematoxylin
Mounting medium on top of the cover glass

A

The use of too much heat during processing

68
Q

The protocol for a new automated strainer is needed, and now often to change solutions must be decided. The best choice is to rotate the solutions:
Hourly
Daily
Biweekly
Based on slides stained

A

Based on slides stained

69
Q

Microscopic review of H&E stained sections reveal an artifacts known as cornflaking. This is caused by:
Hematoxylin precipitate
Excessive formalin pigment
Slide drying before mouting
Paraffin remaining on the slide

A

Slide drying before mouting

Histotech Exam 3 (14 decks)

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