Question Dans Manuel Flashcards by Catherine Desjardins

L’hématoxyline de Harris est utilisée sur des coupes de tissus pour colorer quel élément cellulaire?
Graisse
Glycogène
Noyaux
Cytoplasme

Noyaux

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Comment se nomme le processus de « maturation» de l’hématoxyline?
Hydrolyse
Oxydation
Mordançage
Réductions

Oxydations

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Comment se nomment les produits chimiques de coloration actifs dans la solution d’hématoxyline
mûre?

Hématéine
Hématine
Hématoxyline
Hémosidérine

Hématéine

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Quelle est l’étape la plus importante dans la coloration régressive à l’hématoxyline?
Le postmordancage dans l’acide picrique
Utilise station d’hématoxyline contenant de la glycérine
Différenciation dans l’alcool acide
Lavage à l’eau après l’hématoxyline

Différenciation dans l’alcool acide

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Pendant la coloration à l’hématoxyline et à l’éosine (H&E), quel peut être le résultat si l’ammoniac
n’est pas complètement éliminée par le lavage ?
Décoloration de l’hématoxyline
Mauvaise coloration à l’éosine
Noyaux sous-colorés
Aspect brumeux sur la coloration complétée

Mauvaise coloration à Éosine

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Laquelle des colorations suivantes nécessite un montage avec un milieu de montage aqueux ?
Fite
Huile rouge O
Grocott
H&E

Huile rouge O

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Lesquels des éléments suivants peuvent être ajoutés à un milieu de montage aqueux pour
empêcher la migration des colorants d’aniline dans le milieu environnant ?
Surcre de canne
Baume du Canada
Glycérine
Gélatine

Sucre de canne

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La coloration de Giemsa est plus satisfaisante si le pH se situe entre quelles valeurs?
5.6 et 6,0
6,0 et 6,4
6,4 et 6,9
7,0 et 7,5

6,4 et 6,9

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Laquelle des solutions d’hématoxyline suivantes doit être utilisée dans les jours qui suivent sa
préparation ?
Harris
Delafield
Gill
Weigert

Weigert

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Quel est l’organite qui provoque une augmentation de la basophilie dans le cytoplasme?
Réticulum endoplasmique rugueux
Appareil de Golgi
Mitichondrie
Lysosome

Réticulum endoplasmique rugueux

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La coloration peut être influencée par le fixateur utilisé.
Vrai ou faux

Vrai

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Le chlorure ferrique est à la fois un mordant et un oxydant.
Vrai ou faux

Vrai

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L’hématoxyline de Gill est une hématoxyline de fer.
Vrai ou faux

Faux

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Une augmentation de la température accroît généralement la vitesse de la coloration.
Vrai ou faux

Vrai

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Lors de la coloration d’un support de lames, on constate que l’eau qui suit les alcools réhydratants devient très trouble. Ceci peut très probablement être corrigé de quelle façon?

Sécher plus complètement les lames suivants
Changer le mode de déparaffinage
Changer les alcools
Changer l’eau

Changer les alcools

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Les coupes colorées à l’H&E révèlent des marbrures semblables à des pigments bruns et de rares noyaux noir brillant. Ceci est très probablement dû à un milieu de montage qui :
Est dissous dans le xylène
Est devenu trop épais
A été trop dilué
A été appliqué après avoir laissé sécher la diapositive

A été appliqué après avoir laissé sécher la diapositive

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Les coupes du foie colorées à l’H&E montrent des noyaux très sombres et une certaine coloration bleue du cytoplasme. Ceci est très probablement dû à quoi?
Une quantité importante de Réticulum endoplasmique rugueux
Une coloration excessive avec la solution d’hématoxyline de Meyer
Une différentiation inadéquate de l’hématoxyline
Un séjour trop long dans la solution de bleuissement

Une différentiation inadéquate de l’hématoxyline

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Les coupes de l’intestin grêle colorées au H&E révèlent des muscles, du collagène et des globules rouges tous colorés de la même nuance de rose. Cela indique quoi?
Que la coloration est correcte
Une mauvaise différentiation de l’éosine
Un bleuissement inadéquat avant l’éosine
Que les lames sont restées trop longtemps dans le xylène

Une mauvaise différentiation de l’éosine

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Un précipité bleu-noir est visible sur les coupes colorées au H&E. Ceci pourrait probablement être évité à l’avenir en effectuant quoi?
Filter l’hématoxyline
Diminuer la quantité d’acide dans l’hématoxyline
Augmenter le temps de déshydratation avant le blanchiment
Changer la solution de bleuissement plus fréquemment

Filter l’hématoxyline

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Les sections pour les colorations spéciales ont été accidentellement colorées avec de l’hématoxyline. Pour enlever l’hématoxyline, placez les sections dans quelle solution?
De l’alcool acide
De l’ammoniaque diluée
Du carbonate de lithium
De l’alcool isopropylique

De l’alcool acide

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Hématéine is formed in Mayer hematoxylin solution by the addition of:
Mercuric oxide
Potassium permanganate
Exposure to air
Sodium iodate

Iodate de sodium

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Mordants are used to:
Change the refractive index of the tissue
Link tissue constituents more closely to the dye
Help diffentiate stains
Oxidize staining solutions

Link tissus more closely to the dye

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The combination of a dye and a mordant is called a/an:
Base
Accelerator
Lake
Buffer

Lake

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Mercuric oxide was used in the original formula for Harris hematoxylin to:
Form hématéine
Prevent oxidation
Serve as the mordant
Stabilize solution

Form hématéine

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DNA can be demonstrated with: Éosine Pyronin The Feulgen reaction Fast green

The feulgen reaction

All the following are aluminum Hématoxyline EXCEPT: Gill Harris Mayer Weigert

Weigert

Which of the following Hématoxyline is not readily decolorized with acide soltions? Harris Delafiled Weigert Ehrlich

Weigert

Natural resins are rarely used for mounting sections today because they: Are inherently acidic Dry too fast Must be dissolved in toluene Evaporate from under the coverslip

Are inherently acidic

Which type of metal salt serves as the mordant in Weigert Hématoxyline Iron Aluminium Mercury Thungsten

Iron

Acetic acid is added to Harris Hématoxyline to Keep heterochromatin from staining Make nuclear staining more specific Ripen the hematoxylin Form a dye lake

Make nuclear staining more specific

A dye that may be substituted for hematoxylin in routine staining is Phloxine Methylene blue Carmine Celestine blue

Celestine blue

Which of the following is a good exemple of polychrome stain Alcian blue Congo red Giemsa Kinyoun

Giemsa

The chemical group in dyes that confers the property of color is called a/an Chromogen Chromophore Auxochrome Mordant

Chromophore

An auxochrome is a/an Ionizing group present in dyes Dye group conferring color Negatively charged protein Mordant-dye combination

Ionizing group present in dyes

Which of the following is formed when hematoxylin is subjected to the action of sodium iodate A dye lake Hématéine Hematin A polychrome

Hématéine

Heterochromatin is stained by Hematoxylin Éosine Pyronin Light green

Hématoxyline

If placed in a solution with a pH below the isolelectric point, cytoplasmique proteins will be Basophiles Acidophiles Polychromatic Anionic

Acidophiles

Differentiating in the H&E stain is an exemple of using Excess mordant A weak acid Oxidizers Buffers

A weak acid

A tissu component that takes up a cationioc dye is said to be: Basophiles Acidophiles Chromophoric Absorbant

Basophiles

Into which of the following dye categories does Éosine fall Polychromatic Cationic Anionic Amphoteric

Anionic

For the best cytoplasmic staining the pH of the Éosine should be between 3,0 and 4,0 4,6 and 5,0 5.6 and 6,5 7.0 and 8,0

4,6 and 5,0

Éthylène glycol functions in Gill hematoxylin solution to Oxidize the hematoxylin Mordant the solution Increase the acidity Prevent a surface sheen

Increase the acidity

Which of the following hematoxylin solution will satin the mucin in goblet cells? Gill Mayer Harris Weigert

Gill

Nuclear staining is made more selective by adding which of the following to hematoxylin solutions Ethyl alcohol Chloral acid Ethylene glycol Acetic acid

Acetic acid

Hematoxylin is converted to dye lake in the Mayer formula by adding which of the following to the solution Sodium iodate Citric acid Ammonium aluminum sulfate Chloral hydrate

Ammonium aluminum sulfate

To link hematoxylin to tissu DNA, which of the following must be added Acid Oxidizer Stabilizer Mordant

Mordant

Hematoxylin becomes a dye only when in Is used progressively Is oxidized Influences the isoelectric point of proteins Is polychromed

Is oxidized

For the most transparency and clarity when viewing well-stained microscopic sections, the refractive index of the mounting medium should be Above that of the tissue Near that of the tissue Below that of the tissue Near that of air

Near that of the tissue

A decrease in section transparency can be caused by using Xylene as the solvant for the mounting medium Mounting medium with a refractive Index of 1,51-1,55 Mounting medium that has become too thick Number 1 coverslips for mouting sections

Mounting medium that has become too thick

Fixation with a neutral buffered formalin will cause tissue cytoplasm to Take up more hematoxylin Take up more eosin Be more difficult to blue Resist acid decolonization

Take up more hematoxylin

If the pH of the staining solution is between 4,6 and 5,0 eosin combines with which of the following tissue chemical groups -COO- -NH3+- -PO3- N-N

-NH3+-

True of false Basic dyes have a negative charge

False

True of false Oxidizers are sometimes used for differentiation

True

True or false Eosin is differentiated by the dehydrating alcohols

True

True or false An increase and decrease in the pH of the staining solution can alter staining by changing tissue and/or dye charges

True

True or false Frozen sections can be stained with Toluidine blue O

True

True or false Resinous mounting media have an index of refraction much lower than that of the tissue

False

True or false Resinous mounting media are usually dissolved in toluene, xylene or xylene substitutes

True

True or false The more the better is a good practice for applying mounting resin

False

True or false The nucleolus of plasma cells is stained green with methyl Green-Pyronin technique

False

True or false Bouin solution is a good fixative for tissue to be stained with the Feulgen reaction

False

Microscopic examination of an H&E stained sections shows nuclei with well-defined chromatin patterns, crisp nuclear membranes, and very pale pink staining of the cytoplasm and erythrocytes. These results indicate: Over differentiation with acid alcohol The use of over ripened hematoxylin A probalbloe pH problem with the eosin The propret staining of an H&E

A probalbloe pH problem with the eosin

An H&E stained section shows reddish brown stained nuclei, pink cytoplasm, and bright rose-red erythrocytes. These results indicate: Prolonged dehydration in lower alcohols The use of over ripened hematoxylin A probable pH problem with the eosin Overtreatement with ammonium hydroxide

The use of over ripened hematoxylin

H&E stained sections show very uneven staining, with some areas of the slide well-stained and the other areas unstained. These results indicate that most likely the: Staining solutions were too dilute Sections were cut too thin Paraffin was not completely removed Sections were over fixed

Paraffine was not completely removed Sections

Water bubbles are seen microscopically on H&E stained sections. This could probably be prevented in the future by: Drying the slides completely before beginning the stain Changing the hydrating alcohols more frequently Making sure that the dehydration steps is complete Increasing the time in the last xylene step before coverslipping

Making sure that the dehydration steps is complete

Microscopic examination of an H&E stained sections of kidney is very difficult. Some areas of the tissue cannot be brought into focus, while other areas show excellent detail. This is most likely due to Incomplete dehydration before mounting Mounting medium on top of the glass cover Air bubbles trapped during flotation Too much heat during processing

Mounting medium on top of the glass cover

H&E staining sections show hazy blue nuclei, but recuts from tissue processed 1 week previously and stained in the same basket show excellent nuclear staining. One possible cause of the problem is: The use of too much heat during processing Incomplete dehydration before mounting The use of overripened hematoxylin Mounting medium on top of the cover glass

The use of too much heat during processing

The protocol for a new automated strainer is needed, and now often to change solutions must be decided. The best choice is to rotate the solutions: Hourly Daily Biweekly Based on slides stained

Based on slides stained

Microscopic review of H&E stained sections reveal an artifacts known as cornflaking. This is caused by: Hematoxylin precipitate Excessive formalin pigment Slide drying before mouting Paraffin remaining on the slide

Slide drying before mouting