ques

Question 1

Tissue sources of Acid phosphatase is
1. Prostate
2. Liver
3. Erythrocyte
4. Platelet
5. Kidney

Answer 1

→ 1,3,4

Question 2

• LD isoenzymes maybe useful in determining the source of injury in combination of isoenzymes H & M in following order.

Answer 2

→ 1 > 2 > 3 > 4 > 5

Question 3

In Cherry Crandall Method for Lipase analysis what is the substrate used?

Answer 3

→ Triolein

Question 4

The optimum pH for alkaline phosphatase is

Answer 4

→ 9-10

Question 5

The following is/are TRUE about Endpoint reaction
1. Measurement is made of the amount the reaction has occurred.
2. It is based on the final absorbance reading.

Answer 5

→ Both 1 & 2

Question 6

The following is/are TRUE about Kinetic reaction
1. Measurement is made of the amount the reaction has occurred.
2. It is based on the specific time interval reading.

Answer 6

→ 2 only

Question 7

These enzymes catalyzes the removal of groups from substrates without hydrolysis. The product contains double bonds.

Answer 7

→ Lyases

Question 8

Hydrolysis of ether and ester
1. Lipase
2. ACE
3. SGOT

Answer 8

→ 1 & 2

Question 9

Enzyme is proportional to rate.

Answer 9

→ Zero-order kinetics

Question 10

Identify the image. (dat naka pro pala pag mag aattach ng pic dito so meaning nalang)

takes place when an enzyme inhibitor binds only to the complex formed between the enzyme and the substrate (the E-S complex)

Answer 10

→ Uncompetitive inhibitor

Question 11

In other methods for ACP, storage at room temperature results in loss of enzyme activity.

Answer 11

→ True

Question 12

________ is the only enzyme in which decrease is clinical significance.

Answer 12

→ Pseudocholinesterase

Question 13

Which is/are TRUE about the Karmen Method for SGOT determination
1. Aspartate + α-ketoglutarate → oxaloacetate + glutamate
2. LDH is used as coupling enzyme

Answer 13

→ 1 only

Question 14

In other methods for ALP, low results are due to improper anticoagulant, while high results is due to hyperthyroidism

Answer 14

→ True

Question 15

Cherry Krandal Method uses triolein as a substrate. Fatty acid is measured by increased turbidity.

Answer 15

→ First statement is correct. Second statement is incorrect.

Question 16

Major sources of Gamma-glutamyl transferase includes the following:
1. Heart
2. Liver
3. Kidney
4. Pancreas

Answer 16

→ 2, 3, 4

Question 17

This is also known to its older terminology as Serum Glutamic oxaloacetic transaminase.

Answer 17

→ AST

Question 18

__________ : liberation of carbon dioxide from acidic acid;
_________ : decrease in pH from acetic acid

Answer 18

→ Manometric ; Electrometric

Question 19

Kinetic Method has multiple measurement of absorbance change made at specific time interval.

Answer 19

→ True

Question 20

ALT determination involved LDH as coupling enzyme. Diagnostic significance is for the a specific evaluation of RBC disorders and skeletal involvement.

Answer 20

→ First statement is correct, second statement is incorrect

Question 21

Lactate Dehydrogenase has the highest activity in the heart, liver, skeletal muscle, kidney, and erythrocytes.

Answer 21

→ True

Question 22

Plate Assays for lipase determination uses tweens or tributyrin that change the color of phenol red after complexation with fluorescent dye Rhodamine B.

Answer 22

→ True

Question 23

Ptyalin : ___________ ; Amylopsin : __________

Answer 23

→ Salivary ; Pancreatic

Question 24

Amylase is falsely elevated upon administration opiates as painkiller. Pancreatic amylopsin secreted from the acinar cells of the pancreas.

Answer 24

→ Both statements are correct.

Question 25

Gamma-glutamyl transferase is the smallest enzyme.

Answer 25

→ False

Question 26

Amylase breaks down Starch and Glycogen.

Answer 26

→ True

Question 27

Lactate Dehydrogenase is widely distributed in tissue.

Answer 27

→ True

Question 28

This substance is poisonous when inhaled, swallowed or when absorbed through skin produced during the reaction under reagent preparation and stability.

Answer 28

→ p-nitrophenol

Question 29

Pyridoxal Phosphate (Vitamin B12) is the coenzyme for the AST reaction.

Answer 29

→ False

Question 30

Is the energy required to raise all molecules to the transition state in a chemical reaction so that products may be formed?

Answer 30

→ Activation energy

Question 31

Hemolysis should be avoided because hemoglobin inhibits amylase activity.

Answer 31

→ False

Question 32

CK is stable due to oxidation of sulfhydryl groups.

Answer 32

→ False

Question 33

Which is/are TRUE about Pseudocholinesterase determination:
1. Manometric method liberation of carbon dioxide from acetic acid
2. Cherry Krandall Method is the reference method
3. Decreased level is clinically significant

Answer 33

→ 1 & 3

Question 34

PChe : ___________ ; Ache : _______________

Answer 34

→ White matter of nervous system ; Brain

Question 35

Which is/are TRUE about Reitman and Frankel Method?
1. Color reaction is measured at 505nm due to diazonium salt coupled with keto acid.
2. 35 U/L – Within reference range

Answer 35

→ 1 only

Question 36

__________ : bind to active site ; ______________ : bind to ES complex

Answer 36

→ Competitive ; Uncompetitive

Question 37

Which among the following is a method of analysis for Lipase.
1. Amyloclastic
2. Chromogeninc
3. Saccharogenic
4. Titrimetric

Answer 37

→ None among the options

Question 38

__________ : removal of sialic acid ; ___________ : bind other isoenzymes

Answer 38

→ Neuramidase ; Wheat Germ Lectin

Question 39

Amylopsin is secreted from the Acinar Cells of the pancreas

Answer 39

→ True