Pyruvate Dehydrogenase Mechanism

Question 1

What does dihydrolipoyl transacetylase (E2) catalyze?

Answer 1

It catalyzes the transacetylation of acetyl to acetyl CoA

Question 2

Which reactions occur in the pyruvate dehydrogenase complex (PDC)?

Answer 2

decarboxylation, transacetylation, and dehydrogenase

Question 3

where is the PDC located?

Answer 3

in the mitochondrial matrix

Question 4

under what conditions is PDC used?

Answer 4

aerobic!

Question 5

without oxygen, where does pyruvate go?

Answer 5

it remains in the cytosol

Question 6

pyruvate must enter where to undergo what?

Answer 6

must enter the mitochondria to undergo the CAC

Question 7

oxidative decarboxylation of pyruvate is catalyzed by what?

Answer 7

the pyruvate dehydrogenase complex

Question 8

pyruvate dehydrogenase

Answer 8

produces CO2 from pyruvate, which makes the pyruvate now have only two carbons

Question 9

dihydrolipoyl transacetylase

Answer 9

transfers the two carbon unit (or the acetyl group) on to coenzyme A, making acetyl-CoA

Question 10

dihydrolipoyl dehydrogenase

Answer 10

lipoid acid is reoxidized (this enzyme reduces NAD+ to NADH)