Purification of plasmid DNA Flashcards
1
Q
How to separate plasmid DNA and chromosomal DNA
A
exploit differences- size, conformation
2
Q
separation based on size summary
A
lysis in isotonic solution, only disrupts CSM
With Triton X-100 detergent
Centrifuging- debris co-pellets with chromosomal DNA
Cleared lysate of plasmids pipetted out
3
Q
Separation based on conformation summary
A
EtBr Caesium-chloride centrifugation
4
Q
Purification of mRNA
A
Oligo dT chromatography
