Proving That a Gene Exists Flashcards
1
Q
What is genome annotation?
A
Interpreting a DNA sequence by:
- identifying the position of genes and associated genome features
- ascribing function to genes and other features
- Establishing evolutionary history
2
Q
How many genes are there in the human genome?
A
20000-25000
3
Q
How many proteins are produced by the human genome?
A
> 500,000
4
Q
How many ESTs are produced by the human genome?
A
110,000
5
Q
How can one establish whether a gene is real?
A
Identify its corresponding mRNA.
6
Q
What is the trancriptome?
A
Complete collection of RNA produced from a genome.
7
Q
Does every cell transcribe all of the transcriptome?
A
No, only genes needed for that cell are transcribed.
8
Q
What is the density of genes in human DNA?
A
9
Q
How much of the human DNA is regulatory?
A
27%
10
Q
Which two nucleotides are overrepresented in genes?
A
C and G
11
Q
Why are cytosines commonly methylated in vertebrate DNA?
A
Turns genes off by altering chromatin structure.
