Protozoology Flashcards
Nucleus: One
Peripheral chromatin: fine, uniform, beaded appearance
Karysome: small, central, “bull’s eye”
Trophozoites of Entamoeba histolytica/E. dispar
Nucleus: One
Peripheral chromatin: fine, uniform, beaded appearance
Karyosome: small, eccentric
Trophozoites of Entamoeba hartmanni
Nucleus: One
Peripheral chromatin: dark solid ring
Karyosome: large, eccentric
Trophozoites of Entamoeba coli
Nucleus: One
Peripheral chromatin: none
Karyosome: large, irregular, blot like, central
Trophozoites of Endolimax nana
Smallest intestinal protozoan
Trophozoites of Endolimax nana
Nucleus: One; “basket-shaped”, “basket of flowers”
Peripheral chromatin: none
Karyosome: large, central, surrounded by reflactile achromatic granules
Iodamoeba butschlii trophozoites
Cytoplasm: Fine, granular, ground glass appearance (“clean looking”)
Inclusions: RBCs (E. histolytica)
Entamoeba histolytica/E. dispar trophozoites
Cytoplasm: Fine granular
Entamoeba hartmanni trophozoites
Small race E. histolytica
Entamoeba hartmanni trophozoites
Cytoplasm: vacoulated “dirty looking”
Entamoeba coli trophozoites
Cytoplasm: Fine granular, vacuolated
Inclusions: epithelial cells, WBCs
Entamoeba gingivalis trophozoites
Cytoplasm: Granular, vacuolated
Endolimax nana trophozoites
Cytoplasm: Coarsely, granular, heavily vacuolated
Iodamoeba butschlii trophozoites
Motility: Progressive, directional (explosive)
Hyaline fingerlike or blade pseudopod
Entamoeba histolytica/E. dispar trophozoites
Motility: Non-progressive
Entamoeba hartmanni trophozoites
Motility: Non-progressive (sluggish)
Blunt, granularh pseudopod
Entamoeba coli trophozoites
Motility: Moderately active
Varied pseudopodia
Entamoeba gingivalis trophozoites
Motility: Sluggish, non-progressive
Hyaline, blunt or fingerlike pseudopod
Iodamoeba buschlii trophozoites
Nucleus: 1-4
PC: fine, uniform
K: small, central
Cyst of E. histolvtica/E. dispar
Cytoplasm: CB: elongated bars
with blunt rounded
ends (cigar-
shaped, sausage-
shaped, rod-like)
Cyst of E. histolvtica/E. dispar
Shape: Spherical
Cyst of E. histolytica/ E. dispar
Nucleus : 1-4
Cyst of E. hartmanni
CB: elongated bars with blunt
Spherical, rounded ends (bar-like, rice grain)
Cyst of E. hartmanni
Shape: Spherical
Cyst of E. hartmanni (the same with E. histolytica and E. dispar)
Nucleus: 1-8
CB: with pointed
PC: coarse granular, irregular ends (splinter-like.
K: large, eccentrics
Cyst of E. coli
CB: with pointed broomstick, jagged ends
Cyst of E. coli
Shape: Spherical, oval, triangular, other shapes
Cyst of E. coli
***No cyst stage
E. gingivalis
1-4 (cross-eyed cyst, “button hole”
PC: none (comma, coccoid, short curved
K: large, blot-like, central
Cyst of E. nana
CB: none
granules or small oval masses
PC: none
(comma, coccoid, short curvedrods)
E. nana
Shape: Spherical, oval, ellipsoidal
Cyst of E. nana
Nucleus: 1PC: none
K: large, eccentric, with refractile achromatic granules (periendosome)
I. butschlii Cyst
Cytoplasm:
CB: none
Glycogen:
compact, well-defined (dark brown in lodine prep)
I. butschlii Cyst
Shape: Oval, ellipsoidal, triangular, other shapes
I. butschlii Cyst
“swine amoeba”
E. polecki
Cyst is UNNUCLEATED
E. polecki
Found in pigs and monkey
E. polecki
Cyst stage: “Halo” effect: retraction of cytoplasm from cyst wall
Giardia lamblia
- “Falling leaf,” lip-flop” motility
- 4 pairs of flagella
- Sucking disk
- 2 nuclei: “old man with eyeglasses,” “monkey face”
Giardia lamblia
Disease or Diagnosis:
- Gay bowel syndrome
- Leningrad’s curse (traveler’s diarrhea)
- Backpacker’s diarrhea
- Beaver fever
- ST: Enterotest (String test)
Giardia lamblia
Cyst stage:
- Lemon-shaped
- Anterior, hyaline knob (nipple-like)
Chilomastix mesnili
Trophozoite stage:
- Stiff, rotary motility “shepherd’s crook” appearance
- Prominent cytostome
- Spiral grooves across ventral surface
Chilomastix mesnili
Disease/Diagnosis
- Commensal
Chilomastix mesnili
NO cyst stage
Dientamoeba fragilis
Trophozoite stage:
- Amoeboid, “stellate” appearance
- Tetrakarvosome
- Explosive disintegration in water (“Hakansson
phenomenon”)
- Formerlv classified as amoeba
Dientamoeba fragilis