PROTOZOA Flashcards
4 traits of Trophozoite
motile stage
vegetative stage
sensitive
watery stool
Type of stool specimen to prioritize
fluid/ watery stool
4 Traits of Cyst
non-motile stage
resistant
formed stool
INFECTIVE STAGE
special characteristic of E. gingivalis
no cystic stage
habitat is not in large intestine
all amoeba are commensals except
E. histolytica
entamoeba which smaller than E. hitolytica
E. hartmanni
karyosome of E. histolytica
centrally/ bull’s eye
number of nuclei which consider as infective
4 (present in E. histolytica cyst
lfe cycle of E. histolytica
feces (mature cyst) > ingestion of food/water (mature cyst) > GIT > Small intestine (excystation) > Colon (trophozoite) > encystation
3-6x/day of diarrhea is diagnosed as what as what kind of amoebiasis
amoebic diarrhea
how often the diarrhea to consider acute amoebic dysentry
6-8x/day
extraintestinal amoebiasis occur in organs of:
cerebral (brain) cutaneous (dermal?) pulmonary (lungs) genital hepatic (liver)
stains for trophozoite
QUENSEL’s solution
Buffered methylene blue
stains for cyst
lugol’s iodine
Di antonis for cyst
culture for E. histolytica
LES (Locke’s Egg serum)
RES (Rice Egg saline)
Nutrient Agar saline
Boeck’s and Drbohlav’s
test that differentiating the E. histolytica and dispar
PCR
WHAT ARE THE STAINED SMEAR?
PAS
Chlorazol Black E
Iron hematoxylin
Trichrome
a trophozoite with clean looking cytoplasm with ingested RBC
E. histolytica
smallest protozoa
E. nana
Trophozite of Iodamoeba butschlii
movement: slow
cytoplasm: large glycogen vacuole
Cyst of Iodoamoeba butschlii
large glycogen vacuole brownish red stained by iodine solution
the oral parasite
E. gingivalis
traits of E. gingivalis
movement:
inclusions:
vigorous and fast (multiple pseudopodia)
ingest WBC
E. histolytica is an intestinal amoeba which is not a ____
commensal