Proteins IV Flashcards
Red meat does not include ______
poultry
Most of the meat consumed is from _____ animals because strengthening of the muscles is due to _______ animals
young
older
The red to reddish pink color of red meat is due to ______
myoglobin
______ is the oxygen storage protein in red meat
myoglobin
Red meat is mainly composed of _____ muscle.
skeletal
Muscle condition of the live animal is related to the animal’s __________
level of activity
Water accounts for over ____% of lean tissue by weight. The water is either ______ to _______ or free
70
bound to proteins
free (trapped within muscle cells)
While the animal was alive, the “____” water served as the medium in which nutrients and other solutes were dissolved and transported.
free
In proximate analysis of meat, protein content is typically derived from the determination of muscle ________ content by Kjeldahl analysis
nitrogen
The average ______ content is 16% of protein
nitrogen
As much as 10% of muscle nitrogen originates from __________, such as _______
non-protein sources
nucleic acids/amino acids
The true protein content of red meat is somewhat (higher/lower) than reported
lower
______ can account for 1-13% of fresh weight of separable lean tissue
Lipids
Minimal values (0.5-1%) represent the _____________ present in living cells
membrane phospholipids
Larger lipid values indicate the presence of variable amounts of __________, which are primarily ________
neutral storage lipids
triacylglycerols
The large variability in the percentage of lipid content is mostly accounted for by variability in _______ content
water
______ refers to the inorganic residue remaining after a muscle sample is incinerated at extremely high temperatures and represents the _____ component of muscle.
Ash
mineral
______, ______, and _______ make up the remaining 1% of skeletal muscle.
Carbohydrates, vitamins, and other soluble organic constituents
The main carbohydrate in muscle is ______, which is _____-term energy storage
glycogen
short
The main carbohydrate in meat is _____ because during the conversion of muscle to meat the ______ is largely converted to ______ by _________
lactate
glycogen
lactate
anaerobic glycolysis
Proteins are separated on the basis of __________
solubility
What are the three classes of proteins?
Sarcoplasmic
Myofibrillar
Stromal
________ make up 30% of total muscle protein, and is soluble
Sarcoplasmic proteins
________ make up 50-60% of total muscle protein; solubilized in concentrated salt solutions (e.g., 0.3M NaC1)
Myofibrillar proteins
________ make up 10-20% of total muscle protein; insoluble
Stromal proteins
Sarcoplasmic proteins are dissolved in the _______ inside muscle cells
cytoplasm
Sarcoplasmic proteins, such as _______ and _____ enzymes, are mainly involved in the __________________
myoglobin
glycolytic enzymes
regulation of cell and energy homeostasis
The colors of fresh, cooked, and cured meat products are due, in large part, to the content and the ________ of the ______ associated with myoglobin
oxidation state
iron
Myoglobin content tends to be in lower abundance in ______ animals, hence the pale color of ____ compared to that of ______
young
veal
adult beef
_____ constitute the largest fraction (50-60%) of muscle proteins
Myofibrillar proteins
In muscle tissue, myofibrillar proteins are assembled into complex _______ structural filaments that _____________
quaternary
regulate, support, or perform the mechanical work of contraction.
What are the three types of major myofibrillar proteins in skeletal muscle?
- Contractile
- Regulatory
- Cytoskeletal
_____ and _____ together account for about 40% of the total protein in skeletal muscle.
Myosin
actin
The behavior of _________ proteins is important in fresh meats, because the extent of contraction and rigor mortis development are critical in the successful conversion of muscle to meat, as well as in processed meats
myofibrillar
_________ proteins form the fibrous connective tissues that strengthen and protect muscles
Stromal
_______ proteins contribute to meat toughness
Stromal
_______ is the most abundant protein in the animal body
Collagen
Collagen is a ______ protein
stromal
_______ makes a small contribution to total skeletal muscle protein, but it is the most abundant protein in the animal body
Collagen
Processing and cooking methods for meat are designed to disrupt and partially solubilize ________ fibers
collagen
What are the sources of non-protein nitrogen?
Creatine, amino acids, nucleic acids, and nucleotides
The total content of __________ only accounts for 1.6% of the wet weight of the muscle
non-protein nitrogen
________ and ________ are key components in the development of rigor mortis
Creatine
Nucleotide ATP
Parallel arrangement of elongated multinucleated cells are called ______
myofibers
Skeletal muscles are composed of _______
myofibers
T or F: Individual myofibers range from um to the entire length of the muscle
True
Each myofiber is encased in a layer of connective tissue called the
___________.
endomysium
Groups of myofibers are organized into primary and secondary bundles of _______ that are segregated by another layer of connective tissue called the ________.
fascicles
perimysium
A final layer of heavy connective tissue sheaths, called the __________, surrounds the whole muscle
epimysium
_________ merge with connective tissue tendons to link muscle to the bones
epimysium
The _______ and ________ are infiltrated in the muscle
nervous
vascular
The ___________ and the _________ combine to provide the necessary framework for maintaining the structural integrity of these tissues within the muscle.
perimysium
endomysium
____________ may also be found embedded in the __________ and is visible in red meats as while flecks of fat (marbling) in contrast to the red background of myofibers.
Adipose tissue
perimysium
The abundance of ________ is often used as an indicator in visual appraisal of meat quality.
marbling
Muscle contraction is triggered by increased _________ ________ concentrations
intracellular
calcium
The plasma membrane of myofibers is called the __________
sarcolemma
The _______ penetrate into the myofiber, and form a network of ____________, distributed along the length of the myofiber, and conduct the stimulus for contraction into the interior of the myofiber
sarcolemma transverse tubules (T-tubules)
The T-tubules are in physical contact at periodic intervals with the _____________, which is the muscle equivalent of an endoplasmic reticulum
sarcoplasmic reticulum
The sarcoplasmic reticulum is in physical content at periodic intervals with the ________
T-tubules
The muscle equivalent of the endoplasmic reticulum is the _________
sarcoplasmic reticulum
The _________ functions as a reservoir of calcium ions that serve as the trigger for muscle contraction
sarcoplasmic reticulum
The sarcoplasmic reticulum serves as a reservoir of ______
calcium
Some proteins in the interior of the SR bind _______ ions while the muscle is at rest
calcium
Other proteins in the SR open in response to electrochemical stimulus, allowing diffusion of _____ ions from the _____ to the ________
calcium
SR
sarcoplasm
What triggers muscle contraction?
Diffusion of calcium ions from within the SR to the sarcoplasm
What triggers muscle relaxation?
Protein pumps calcium back into the lumen of the SR during relaxation
___________ serve as energy transducers for the myofiber
Mitochondria
_________ serve as a major reservoir for a family of proteolytic enzymes known as _______
Lysosomes
cathepsins
_______ play a catabolic function in protein turnover
cathepsins
Myofibers are typically ____nucleated
multi
Where are the nuclei of myofibers?
Dispersed; typically found beneath the sarcolemma
The sarcoplasm may contain ______ particles and ______ droplets
glycogen
lipid
The oxygen storage, enzyme levels, metabolic intermediates are found to varying degrees within the __________
sarcoplasm
What are the 3 types of myofibers?
1) Slow-red
2A) Fast-red
2B) Fast-white