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Molecular Biology (Bio 204) > Proteins (and some other things)for Exam 2 > Flashcards

Proteins (and some other things)for Exam 2 Flashcards

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1
Q

Type 1A topoisomerases

A

useful in taking 2 DNA circles apart in bacterial replication termination
can cut ss regions, DNA pol fills in the gap (ATP required)

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2
Q

SeqA

A

method of refiring regulation in bacteria
when hemi-methylated, origin is blocked by SeqA (delays methylation)
(blocks origin to delay methylation)

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3
Q

TERT

A

in eukaryotes
protein component of telomerase

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4
Q

Telomerase

A

maintains telomere in euks
made up of 2 components (TERT and TERC)

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5
Q

DNA pol 𝛿

A

Eukaryotic lagging strand polymerase
not part of replisome

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6
Q

RFC

A

eukaryotic clamp loader

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7
Q

RNA pol I

A

in eukaryotes
ribosomal RNA pol

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8
Q

ASF1

A

helps FACT in moving nucleosomes out of the way in eukaryotic replication

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9
Q

-35 box, -10 box

A

in bacteria, promoter elements
-35 consensus sequence is TTGACA
-10 consensus sequence is TATAAT
“consensus is best”

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10
Q

Tau subunit (s)

A

binds itself + DNA pol III (and clamp loader and helicase) which allows coordination of leading and lagging strands in bacterial DNA rep elongation

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11
Q

DNA pol III

A

main replication enzyme in bacteria

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12
Q

DnaB

A

helicase in bacteria
breaks H bonds between bases

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13
Q

RNA pol II

A

in eukaryotes
main/most complicated one we focus on
all mRNAs, some regulatory RNAs

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14
Q

DnaC

A

Helicase (DnaB) loader in bacteria

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15
Q

RNA pol III

A

tRNA, ssRNAs, regulatory RNAs

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16
Q

Fen1

A

endonuclease in eukaryotes that cuts of “flap” after pol (𝛿) peels back the DNA that includes the primer

17
Q

Type 2 topoisomerases

A

useful in taking 2 DNA circles apart in bacterial replication termination
can make double stranded cuts (requires ATP)

18
Q

Type 1B topoisomerases

A

useful in taking 2 DNA circles apart in bacterial replication termination
cuts 1 strand and lets it unwind (no ATP required)

19
Q

TFIIE, TFIIF

TFIIH also exists

A

one of the multiple proteins eukaryotes require to recognize the many different promoter elements (BRE-TATA-INR-DPE-etc)
help pol II and TFIIH bind

20
Q

DNA pol 𝝴

A

eukaryotic leading strand polymerase

21
Q

DNA pol I

A

in bacteria, helps with primer removal
binds 3’ OH and removes some of the new strand (including the primer), synthesizing where it goes

22
Q

TERC

A

in eukaryotes
RNA template component of telomerase

23
Q

Ter

A

in bacteria
DNA sequences that bind Tus proteins

24
Q

𝞼 subunit

A

in bacterial txn
binds core enzyme (𝛂2, β, β’) to make holoenzyme
part that recognizes promoter
doesnt bind DNA unless attached to core enzye
several types of 𝞼 subunits - good for cell because they can bind promoters in diff conditions (diff consensus sequences)
binds -35 and -10 box

25
Q

Cdt2 and Cdc6

A

in eukaryotes part of licensing
help MCM binds
are degraded after this

26
Q

DDK

A

in eukaryotes, part of firing of origins
a kinase regulated by Cdk
phosphorylated + thus activates

27
Q

TFIIA, TFIIB, TFIID

A

transcription factors involved in DNA binding
one of the multiple proteins eukaryotes require to recognize the many different promoter elements (BRE-TATA-INR-DPE-etc)
D recognizes TATA, INR
B recognizes BRE
A helps
the 𝞼 equivalents in eukaryotes

28
Q

ORC

A

Origin recognition complex
(eukaryotes)
made up of 6 polypeptides

29
Q

TATA box

A

in eukaryotes
TATAAAA ——25bp—-+1

30
Q

DNA pol 𝛂

A

works with primase in eukaryotes to synthesize primer - adds few nucleotides for initiation

31
Q

FACT

A

histone chaperone
in eukaryotes
FAcilitates Chromatin Transcription
binds H3:H4 dimer + moves it behind replisome with help of ASF1
interacts with CMG helicase and DNA pol 𝛂 at fork

32
Q

MCM complex

A

helicase for eukaryotes in replication
unwinds origin

33
Q

DnaA

A

origin binding protein in bacteria
DNA coils around it
either bound to ATP (associated) or ADP (dissociates)

34
Q

DAT sites

A

in bacteria regulation of origin refiring
DAT sites bind DnaA:ATP with higher affinity than origin
so when more of them (after replication there are 2) titrate out DnaA:ATP

35
Q

DnaN

A

B-clamp (sliding clamp) in bacteria

36
Q

DnaG

A

primase in bacteria

37
Q

Tus

A

In bacteria
termination factor - orientation of these proteins dictate direction DNA pol III can move

38
Q

PCNA

A

Sliding (Beta) clamp for eukaryotes

39
Q

TATA binding protein (TBP)

A

the one protein required for transcription initiation by all eukaryotic polymerases
a subunit of TFIID

Molecular Biology (Bio 204) (9 decks)

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