Protein Synthesis - Transcription Flashcards
what is transcription
info encoded in DNA copied into complementary RNA
Promotor
sequence that lies just before a gene
serves as a signaling sequence, marking the starting point and direction for RNA polymerase to begin transcribing a gene
Promotor in Eukaryotes
TATA box –> hydrogen bonds are easier to break
Promotor in Prokaryotes
TATAAT sequence
What is enlongation
RNA polymerase makes a complimentary copy in the 5’–> 3’, using the 3’ to 5’ as template
Examples of termination
Termination code
in Prokaryotes - bind to mRNA + stop it
mRNA binds with itself
RNA poly binds to another promotor
Post-transcriptional modifications
Poly A Tail
5’ Cap of 7 guanine
cut introns using snRNPs + mRNA (complex if spliceosome
What is alternative splicing
cells can choose different combinations of exons from the same pre-mRNA to make a final mRNA –> why humans can produce 100 000 proteins from 20 000 genes