Protein Structure I and II

Question 1

Describe the geometry of the peptide bond.

Answer 1

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Question 2

Indicate the properties of of the peptide bond.

Answer 2

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Question 3

Be able to define the following terms: multimeric, prosthetic group, native conformation, loops, bends, motif and domain.

Answer 3

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Question 4

Describe the levels of protein structure: primary, secondary, tertiary, quaternary.

Answer 4

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Question 5

Describe the structure of the α-helix and the β-sheet.

Answer 5

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Question 6

Identify the bonds that stabilize the structure of α-helix and the β-sheet

Answer 6

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Question 7

Identify amino acids that can disrupt the α-helix

Answer 7

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Question 8

Describe what constitutes “supersecondary” structure

Answer 8

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Question 9

Differentiate between the structure and function of fibrous and globular proteins.

Answer 9

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Question 10

Explain the structure of silk as a protein that is predominantly composed of β-sheet

Answer 10

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Question 11

Describe the bonds that stabilize tertiary and quaternary structures.

Answer 11

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Question 12

List the advantages of quaternary structure.

Answer 12

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Question 13

Describe protein denaturation and list some common denaturing agents.

Answer 13

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Question 14

Briefly describe the role of chaperones

Answer 14

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Question 15

Describe the sequencing of a polypeptide by Edman degradation

Answer 15

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Question 16

Compare and contrast the structure and function of myoglobin and hemoglobin

Answer 16

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