Protein Quantification Using Colorimetric Detection

Question 1

The reagent used for protein detection

Answer 1

Bio rad

Question 2

A dilution series is prepared containing

Answer 2

Dilution series from 2 mg / ml bs A stock solution and PBS

Question 3

General equation for calculating volume required

Answer 3

V1= (v 2xc2)/c1

V1: volume of stock solution required (ml)
V2: final volume (ml)
C1: initial stock concentration
C2: final concentration required

Question 4

What is the absorbency measured at?

Answer 4

595 Nm