Protein production

Question 1

What’s two applications of plaque assays?

Answer 1

1. Determine virus titer
2. Isolation of single baculovirus clones (pick a colony from a plaque).

Question 2

What are the differences between bdded viruses and occlusion-deriveed viruses?

Answer 2

ODVs contain multiple genomes and perform the primary infection as well as further ones.

BVs perform secondary infections, they contain a single genome.

Question 3

Pros and cons of transpostion-based BEVS?

Question 4

Key steps of protein production?

Answer 4

1. cDNA synthesis
2. Choice of expression system
3. Protein purification
4. Protein charcterisation

Question 5

What are the key steps of a directed evolution experiment?

Answer 5

1. Generate mutant protein library. Insert in organism.
2. Select mutant w/ best characteristic
3. Do again

Directed evoltyuion always yield a result, most often it will be costly in form of fitness.