Protein Hydrolysis Flashcards

0
Q

What are exoenzymes capable of?

A

Hydrolyzing large proteins.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
1
Q

What metabolic activity is protein hydrolysis?

A

Polymer hydrolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What are large proteins?

A

Polymers of amino acids.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What are the end products of hydrolyzed large proteins?

A

Soluble end products…

  1. Peptones
  2. Proteoses
  3. Peptides
  4. Amino acids
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What are the smaller fragments used for after the protein is hydrolyzed?

A

Used in cell building material or for energy.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What are the two enzymes capable of degrading proteins for this lab?

A
  1. Gelatin

2. Casein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Where does gelatin derive from?

A

A typical animal protein, derived from collagen found in hide and connective tissues of animals.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What type of protein is casein?

A

An animal milk protein.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

At what temperature is gelatin a solid?

A

20 degrees C (rm temp.)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

At what temperature does gelatin melt?

A

28-30 degrees C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Describe a negative gelatin result (protein is unhydrolyzed).

A

Gelatin remains, protein precipitates, forming a cloudy white turbidity.
E. Coli was negative.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Describe a positive gelatin test (protein is hydrolyzed)

A

There will be a well created where the medium was liquified.
Serratia marcescens was positive (turns a bit red).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

In reference to casein what is peptonization?

A

When an organism is capable of digesting casein; causes a clearing of milk…

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Describe a positive casein test (protein is hydrolyzed).

A

There will be a clear zone around the growth.

Bacillius ceresus was a positive test.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Describe a negative casein test (protein is unhydrolyzed).

A

Area will remain opaque.

E. Colin was negative.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What are incubation requirements for casein?

A

Plate at 32 degrees C for 48-72hrs

16
Q

What are the incubation requirements for gelatin?

A

Plate at 37 degree C for 48-72hrs

17
Q

What scientist is responsible for the methods we used in this lab?

A

Fraser

18
Q

What are proteases?

A

Exoenzymes that hydrolyze proteins into end products.