Protein Function: Catalysis Flashcards
Lecture 6
What does a catalyst do?
Increases the rate of reaction by lowering the activation energy barrier in the absence of thermal activation
Why don’t reactions occur instantaneously?
Provides an opportunity for regulation; also, reactions in cells are steady state
Does a catalyst influence the rate of the reaction?
Yes
Does a catalyst influence the position of equilibrium of a reaction?
No.
Describe the slope of the rate of a reaction and the concentration of a reagent without an enzyme catalyst.
Linear slope
Describe the slope of the rate of a reaction and the concentration of a reagent with an enzyme catalyst.
Hyperbolic slope, no longer linear
Why would a reaction’s rate plateau when using enzymes in vitro?
If the enzymes become saturated with substrates, then they’re all occupied and can not increase the rate any more.
What’s the first step of catalysis?
Substrate binding
How does a catalyst act?
Forms a reversible complex with a substrate, stabilizing a transition state
What does folding the catalyst do?
Creates a binding site where specific R groups are presented to each other; specific topology allows enzymes to have specificity.
Is Vmax ever reached?
No, it is only approached.
What is Vmax proportional to?
the concentration of enzymes
What is Vmax?
maximum velocity of a reaction, when 100% of the enzymes are bound to a substrate
What determines the initial rate of an enzyme catalyzed reaction?
Km, Vmax, and the initial substrate concentration
What does Vmax tell us?
- At high [S], reaction rates vary significantly among different enzymes.
- Allows a calculation of kcat (the turnover number of a specific enzyme - measures how effective an enzyme is)
- In vivo, few enzymes encounter saturating [S]; Vmax and kcat set upper bounds, enabling estimates of reaction rates in vivo.