Protein Folding II

Question 1

What is used to predict the propensity of the secondary structure?

Answer 1

F= aa in alpha helix/All aa

Propensity= f / all aa

Higher value, higher propensity of Alpha form.
This prediction is 65% correct

Question 2

PDI

Answer 2

Protein disulfide isomerase is an accessory enzyme that rearranges the polypeptide’s non-native S-S bonds

It binds to a polypeptide and stays intact until proper disulfide bonds are formed

Question 3

PPI

Answer 3

Peptidyl prolyl cis trans isomerase

Helps protein maintain cis configuration for stability

Question 4

Molecular chaperones ____ and ____ are ____ driven.

Answer 4

HSP 70, HSP 30, ATP

Question 5

HSP 70 function

Answer 5

Reverses misfolds, unfold/fold trafficked proteins; folds newly synthesized proteins

Question 6

HSP 90 function

Answer 6

Utilized for signal transduction

Question 7

Mitochondria have special Hsps ____ and ____.

Answer 7

Hsp 60 and Hsp 70 that function in the cytosol

Question 8

Small Hsp include

Answer 8

HSP27 and beta-crystallin

Question 9

How can proteins be denatured? (3)

Answer 9

heat, ph and agitation

Question 10

Chemicals like SDS detergents denature proteins by ___ while Chaotropic agents and Organic solvents denature proteins by ____.

Answer 10

breaking S-S bridges; Breaking H-bonds

Question 11

Chaotropic agents

Answer 11

Urea, guanidine hydrochloride

Question 12

Organic solvents

Answer 12

TCA: tricholoracetic acid

Question 13

19S portion of 26S proteasome function is to

Answer 13

recognize ubiquinated proteins which is cleaved by isopeptidase and sent to 20S

Question 14

20S portion of 26S proteasome function is to

Answer 14

“catalytic core” or sealed barrier

Question 15

___ and ___ are chaperonins

Answer 15

Hsp 60 and Hsp 10