Proper Processing of Fresh Tissue

Question 1

Factors affecting the method to be used during Histopathologic Examination

Answer 1

Structure or Inclusions
Amount and Nature of the tissue
Urgency of Investigation

Question 2

For rapid microscopic analysis while surgery is taking place

Answer 2

Fresh tissue examination

Question 3

Fresh tissue examination methods

Answer 3

Teasing
Crushing
Smear Prep
Frozen Sections

Question 4

Preferred in many conditions for more accurate diagnosis

Answer 4

Fixed tissue examination

Question 5

Allows the examination of tissue in its living state

Answer 5

Fresh tissue examination

Question 6

Done by carefully separating very thin tissue slices with dissecting needles

Answer 6

Teasing / Dissociation

Question 7

Stain for Teasing

Answer 7

Methylene Blue

Question 8

Done by simply crushing small pieces of tissue
between two glass slides or between a glass slide
and a cover slip

Answer 8

Squash Preparation / Crushing

Question 9

Stain for Squash Prep / Crushing

Answer 9

Supravital Stain

Question 10

Method of choice for examining sediments or
samples in liquid/fluid form.

Answer 10

Smear Preparation

Question 11

Smear Prep encompasses the ff. methods of examination

Answer 11

Streaking
Spreading
Pull-apart
Touch Preparation

Question 12

Measurement for frozen sections

Answer 12

5-10um

Question 13

Temperature for frozen sections

Answer 13

-20 to -30C

Question 14

Gel like medium for Frozen sections consist of

Answer 14

Polyethylene Glycol and Polyvinyl Alcohol

Question 15

NSS stands for

Answer 15

Normal Saline Solution

Question 16

During Teasing/Dissociation, tissues are immerse in?

Answer 16

NSS