Prokaryotic Transcription and MEdicine

Question 1

Rifaampicin and Rifamycin B

Answer 1

block 1st phosphodiester bond in RNA chain. Blocks initiation of transcription

Question 2

Cordycepin

Answer 2

adenosine analog lacking 3’OH causing RNA chain termination

Question 3

Streptolydigin

Answer 3

binds to RNA polymerase and prevents elongation

Question 4

Actinomycin D

Answer 4

binds to DNA by intercalating between GC base pairs (research tool only- too toxic for medical use)

Question 5

amanitin

Answer 5

fungal toxin that inhibits RNA poly II with weakly for RNA poly II. insensitive to RNA poly I and mitochondrial RNA polymerase

Question 6

RNA poly I

Answer 6

5.8S, 18S, 28S r RNA

Question 7

RNA poly II

Answer 7

all protein coding genes, plus snoRNA genes, miRNA, siRNA, and most snRNA genes

Question 8

RNA poly II

Answer 8

tRNA genes, 5S rRNA genes, some snRNA genes and genes for other small RNAs