Producing proteins on a large scale 1

Question 1

What is upstream processing?

Answer 1

Processes involved with cells - before the extraction of the protein

Question 2

What downstream processing?

Answer 2

Precesses post cells

Question 3

If a protein is secreted you collect the supernatant. What is the processing that follows this?

Answer 3

Chromatography concentration to purify the protein

Question 4

If your protein is intracellular how do you access it?

Answer 4

Disrupt the cells using emulsification or sonification. The debris is then removed using centrifugation or filtration. the lysate is then processed as in the same way as a secreted supernatant product

Question 5

What is the mass balance equation?

Answer 5

Accumulation = IN - OUT + Generation - consumption

Question 6

Whats is the accumulation in steady state?

Answer 6

zero

In = out

Question 7

What is purge?

Answer 7

Removing something from the bioreactor

Question 8

What is recycling used for?

Answer 8

To conserve a valuable material by reusing it, improve cell conc., achieve steady state faster