Processing

Question 1

Calculate MCV, MCH, MCHC.

Answer 1

Refer to formula chart

Question 2

Calculate Retic count

Answer 2

Refer to chart

Question 3

What is Rule of three?

Answer 3

RBC X 3= HGB X 3= HCT +/- 3

Question 4

Confirmatory testing options for sickle cell disease and trait

Answer 4

Electrophoresis

Question 5

Sources of errors when performing manual microhematocrit

Answer 5

• Undercentrifugation, EDTA blood tube allowed to sit out overnight at RT, Buffy coat included in reading (increased)
• Decreased blood:anticoagulant ratio (decreased)

Question 6

Normal Range for reticulocyte count

Answer 6

O.5-1.5%

Question 7

RBC morphology with Increased Reticulocyte count

Answer 7

Moderate polychromasia and some nRBCs

Question 8

Increased reticulocyte counts are associated with___

Answer 8

Hemolytic anemias

Question 9

Decreased reticulocyte count is associated with ___

Answer 9

Aplastic anemia

Question 10

Number of reticulocyte in a volume of blood (usually either microliter or liter)

Answer 10

Absolute retic count

Question 11

Calculation used to compensate for the increased maturation time of shift retics or nRBCs in the peripheral blood.

Answer 11

Retic production index (RPI)

Question 12

Calculation used when the patient has a decreased hematocrit.

Answer 12

Corrected Reticulocyte count

Question 13

The purpose of the Kleihauer-Betke Stain?

Answer 13

To differentiate HGB A from HGB F

Question 14

The appearance of fetal hemoglobin and A hemoglobin look like following an acid elution test.

Answer 14

HGB F: Dark Pink

HGB A: Ghost cells

Question 15

Normal range for Kleihauer-Betke Stain

Answer 15

</= 2%

Question 16

Diagnostic value of an erythrocyte sedimentation rate.

Answer 16

How far red cells fall in mm per hour

Question 17

Three stages that occur during the 60 minutes waiting period in ESR.

Answer 17

Rouleaux-10 mins
Rapid fall-40 mins
Packing -10 mins

Question 18

Normal ESR value for men and women.

Answer 18

Women: 0-20 mm/hr
Male: 0-15 mm/hr

Question 19

Decreased ESR is due to____

Answer 19

Microcytes, Spherocytes, sickle cells, significantly delayed in testing

Question 20

Increased ESR is due to ____

Answer 20

Rouleaux, Acute phase reactants (such as certain types of plasma proteins)

Question 21

ESR is a nonspecific measure of

Answer 21

inflammation and tissue damage

Question 22

Principle of the screening test of hemoglobing S.

Answer 22

Hemoglobin S is insoluble in a reducing agent, such as sodium dithionite, causing a turbid solution.

Question 23

Three causes of a false positive sickle cell screening test.

Answer 23

Proteinemia, >18 g/dL HGB, Lipemic plasma, other sickling HGB

Question 24

Three causes of a false negative sickle cell screening test.

Answer 24

<7 g/dL HGB, Multiple transfusion, testing newborns

Question 25

Hemoglobin Electrophoresis for sickle cell anemia

Answer 25

S>F (no A)

Question 26

Hemoglobin Electrophoresis for Sickle cell Trait (AS)

Answer 26

A>S

Question 27

Probable cause of WBC being 99.9 and H and H in balance.

Answer 27

Leukemia patient

Question 28

How would you report out a valid CBC result with leukemia patient.

Answer 28

WBCs dilute with saline and Microhemocritic (packed RBCs)

Question 29

Probable cause of very high MCV, RBC low, H & H not balance.

Answer 29

Cold agglutinin

Question 30

How would you report out a valid CBC result with cold agglutinin patient?

Answer 30

Look at the smear for clumps, then warm at 37 degree water bath then re try.

Question 31

Probable cause of high MCHC, H & H not balance.

Answer 31

Lipemic plasma

Question 32

How would you report out a valid CBC result with Lipemic plasma.

Answer 32

Confirm on microhemocritic- if it is milky then suspect lipemic plasma. and Want do plasma blank to correct hemoglobin

Question 33

Reason for the WBC correction for the presence of nRBCs.

Answer 33

WBC will be falsely elevated.

Question 34

Guideline to follow when WBC correction is done.

Answer 34

>5 nRBCs

Question 35

Formula for WBC correction.

Answer 35

WBC X100/ nRBCs+100