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Microbiology > Procedures > Flashcards

Procedures Flashcards

1
Q

ALA DISK

A 
For Haemophilus
Porphobilinogens and porphyrins
X-independent strains
Delta-amino levulenic acid
\+ H. Paraflu and H. Parahaem
- H. Flu
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2
Q

Bacitracin

A

Taxo “A”
S-Group A
R-Group B

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3
Q

Beta-lactamase Disk

A

N.G.
Moraxella catarrhalis
H. Flu

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4
Q

Bile Esculin

A

Esculin and ferric citrate and 4% oxbile

+Enterococcus Faecalis
-Streptococcus agalactiae

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5
Q

Bile solubility

A

Desoxycholate reagent
+Streptococcus pneumoniae
-Streptococcus salivarius

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6
Q

Catalase

A

H2O2 —> H2O + O2
+Staphylococcus aureus
-Streptococcus pyogenes

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7
Q

CAMP

A

Uses Staphylococcus aureus
+Streptococcus agalactiae
-Streptococcus pyogenes
Shovel shape

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8
Q

Catarrhalis

A

Butyrate esterase
Bromo-chloro-indolyl butyrate
+Moraxella catarrhalis (blue-green)
-Neisseria lactamica

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9
Q

Simmons Citrate Agar

A

GN
Green to blue+
+klebsiella pneumoniae
-Escherichia coli

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10
Q

Coagulase

A

Lyophilized rabbit plasma
Rapid slide-bound coagulase
Tube-free coagulase

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11
Q

CTA

Cystine trypticase Agar

A 
N.G. = Glucose
N.M. = Maltose
N.L. = Maltose and Lactose
N.S. = Maltose and Sucrose
Moraxella = -
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12
Q

D Test

A

Clindamycin
+Clindamycin resistance
-Inducible clindamycin resistance
-Constitutive clindamycin resistance

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13
Q

Decarboxylase Broth (Moeller) LAO

A

Lysine
Arginine
Ornithine
Bromcresol purple and cresol red

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14
Q

DNAse Agar

A

Toluidine Blue
+Serratia marcescens and Staphylococcus aureus
-Coag Neg Staph
+Pink to Red Zone or Clearing with Methyl Green

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15
Q

E-Test

A

Gradient Diffusion Method

GN aerobes and Staphylococci, H. flu, and Strep. pneimoniae

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16
Q

Germ Tube

A

Candida albicans

Beginning of true hyphae

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17
Q

Group B Strep Detect and Carrot Broth

A

+Step. agalactiae

Entero. faecalis

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18
Q

Haemophilus Identification Agar (HID I)

A
  1. hemin - X factor
  2. NAD - V factor
  3. XV factors
  4. NAD + Horse blood
    I, II, III, IV - H. aphrophilus
    III, IV - H. flu
    II, III, IV w/beta - H. parahaem
    II, III, IV - H. paraflu
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19
Q

Hippurate Hydrolysis Rapid

A

Sodium hippurate, Hydindantin, ammonia, nihydrin

+Purple = Group B Strep, Gardnerella vaginalis, Campylobacter jejuni

20
Q

Hodge Test

A

Trypticase Soy, Ertapenem disk
+ Klebsiella pneumoniae ATCC BAA-1705
-1706

21
Q

Indole Test

A

Benxyl pyrrol
Tryptophan
+E. Coli (Red)
-Klebsiela pneumoniae

22
Q

Kligler Iron Agar (KIA)

A

Phenol Red
AK/A - Morganella morganii
AK/AK - E. Coli
AK/A - Salmonella sp. (H2S+)

23
Q

Kirby-Bauer Susceptibility

A

Susceptibility
E. coli
Klebsiella pneumonia
Staph aureus

24
Q

LAP Test

A 
Leucine aminopeptidase
DIsk
Leucine and Beta naphthylamide
Turns pink/orange
\+ Entero. faecalis
Aero. viridans
25
Q

Lysine Iron Agar (LIA)

A

Bromcresol Purple, Ferric ammonium citrate and thiosulfate.
K/K - lysine decarboxylation - Klebsiella
K/A - Only glucose fermentation - Salmonella
R/A - Lysine deamination - Proteus/Providencia
K/A H2S+ -Glucose and H2S fermentation - Citrobacter

26
Q

LIM and Carrot Broth

A

Colistin and nalidixic
+ orange
- no change
+ Beta hemolytic. Catalase Negative GPC

27
Q

Mannitol Salt Agar

A

7.5 Sodium chloride
Phenol Red
+ Staph aureus (yellow)
- CNS

28
Q

MR-VP

A

Methyl Red
5% alpha-naphthol then potassium hydroxide
+- E. Coli
-+ Entero aerogenes

29
Q

Microdase Disk

A

DMSO TMDP into cell
+ blue = Micrococcus
- NC = Staph spp.

30
Q

Motility Indole Ornithine (MIO)

A

Tryptophanase and Bromcresol purple
+++ E. coli
— Klebsiella pneumoniae

31
Q

Motility Agar

A

+ E. coli

- Klebsiella pneumoniae

32
Q

MRSA and Vancomycin

A 
Methicillin resistant Staph Aureus with Oxicillin
 Sometimes Chromogenic Agar for Both
VRE Vancomycin Resistant Enterococci 
Growth is resistance
NG is sustibility
33
Q

6.5% NaCl

A

+ Enter. Spp

- Group D Strep

34
Q

Nitrate Test

A

Nitrate to Nitrite
Sulfanilic acid and N,N-dimethyl-alpha-naphthylamine
+ E. coli
- Acinetobacter baumannii

35
Q

Novobiocin Disk

A

S - Staph epi

R - Staph saprophyticus

36
Q

Optochin Disk

A

Eythlhydrocupreine hydrochloride 5ug
+ Step pneumoniae
- Virdans strep and Entero. faecalis

37
Q

Oxidase

A

Indophenol oxidase Pink/Purple +
+ Psudomonas aeruginosa
- E. coli

38
Q

Oxidative Fermentation

A 
Bromthymol blue makes green to yellow
Open tube/Oil
Oxidative A/NC
Fermentative A/A
Neither NC/NC
39
Q

Phenylalanine Agar (PDA)

A

Phenylpyruvic acid

+ is green

40
Q

PYR Test

A

L-pyrrolindonyl-Beta-nephthylamide
+ Entero faecalis or Group A Strep
- Group B Strep

41
Q

Rapid NH

A

Neisseria Unknowns

42
Q

Staphyaurex Latex

A

Protein A

+ Staph aureus

43
Q

Streptex Acid Extraction

A

Agglutination + Lancefield Streptococci

44
Q

Superoxol Catalase

A

N. gonorrhoeae with 30% Peroxide

45
Q

Synergy Quad Plates

A

I: Control Pink
II: Vancomycin 6mg
III: Gentamicin 500mg
IV: Streptomycin 2000mg

46
Q

Urea Agar

A

Phenol red
+ Proteus mirabilis
= E. coli

47
Q

X&V Factor

A

H flu XY

H aphrophilus X or Y

Microbiology (11 decks)

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