Principles of Microscopy Flashcards

1
Q

Cytology

A

Study of cell structure

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2
Q

Light Microscopy

A
  • Passing a beam of light through a specimen
  • maginification up to 1000x
  • resolution 200nm
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3
Q

Electron Microscopy

A
  • passing a beam of electrons over or through a specimen
  • magnification up to 10,000,000x
  • resolution 0.05-2nm
  • 2 main types: scanning (SEM) and transmission (TEM)
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4
Q

Resolution

A

The minimum distance 2 points can be separated and still be distinguishable

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5
Q

Magnification

A

Ratio of an objects image size to its real size

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6
Q

Contrast

A

Difference in brightness between light and dark areas

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