primers Flashcards
1
Q
N-ter His Forward primer
A
5’ junk, Ndel, gene 3’
2
Q
N-ter His reverse primer
A
5’ gene, stop codon, BamHI, junk 3’
3
Q
No tag forward primer
A
5’ junk, Ncol, 2 extra bps, gene 3’
4
Q
no tag reverse primer
A
5’ gene, stop codon, BamHi, junk 3’
5
Q
c-ter his tag forward primer
A
5’ junk, Ncol, 2 extra bps, gene 3’
6
Q
c-ter his tag reverse primer
A
5’ gene, Xhol, junk 3’
7
Q
both N-ter and C-ter his tag forward primer
A
5’ junk, Ndel, gene 3’
8
Q
both N-ter and C-ter his tag reverse primer
A
5’ gene, Xhol, junk 3’
9
Q
junk
A
CAGGTA
10
Q
Ndel
A
CATATG
11
Q
Ncol
A
CCATGG
12
Q
Bamhi
A
GGATCC
13
Q
Xhol
A
CTCGAG
14
Q
EcoRi
A
GAATTC
15
Q
stop
A
TAA, TAG, TGA
