pretest

Question 1

what reagents and patient sample do you use in an antibody screen?

Answer 1

patient serum
check cells
screen cells
LISS

Question 2

what is the purpose of the DAT?

Answer 2

detects Ab and complement attached to RBC’s in vivo (inside the body)

Question 3

what are the components of DAT?

Answer 3

patient cells

AHG

Question 4

If a Rh control tube is necessary in the Rh testing, and it is positive, the interpretation of the Rh type is what?

Answer 4

invalid

Question 5

name and differentiate between the 2 types of AHG

Answer 5

polyspecific: contains both C3d and anti-IgG
monospecific: contains one or the other

Question 6

what is the purpose of the IAT?

Answer 6

detects Abs and complement attatched to RBC’s invitro

Question 7

what are the components of the IAT

Answer 7

Patient serum and screen cells

Question 8

name 2 blood bank procedures that incorporate IAT?

Answer 8

crossmatch

Ab screen

Question 9

the presence of agglutinated cells indicates that the maternal blood contains possibly a significant number of Rh ___________ cells.

Answer 9

positive fetal

Question 10

what term is used to describe that agglutination that constitutes a positive test?

Answer 10

rosettes

Question 11

name the quantitative test performed to detect fetal cells?

Answer 11

kleinhaure betke

Question 12

what other situations may warrant a kleinhaure betke to be performed?

Answer 12

miscarriage and abortion

Question 13

name four condition the DAT test is used to detect?

Answer 13

HDFN
AIHA
HTR
drug induced RBC sesitization

Question 14

list the components of an autocontrol

Answer 14

patient cells

patient serum

Question 15

list the components of a crossmatch

Answer 15

patient serum

donor RBC’s

Question 16

2 cells populations in a patient specimen can produce _____ agglutination.

Answer 16

mixed field

Question 17

what can be done to enhance an ABO reaction that is very weak upon IS?

Answer 17

incubate

Question 18

what blood bank procedure is sued to resolve a positive DAT?

Answer 18

Elution

Question 19

what blood bank procedure is used to resolve a positive antibody?

Answer 19

antibody panel

Question 20

clinically significant antibodies are Ig__ antibodies which like to react at ____ C and in the _____ phase?

Answer 20

G
37
AHG

Question 21

In a pregnant female, what antibody class is clinically significant?

Answer 21

Rh-IgG

Question 22

what are the 2 AABB requirements for routine crossmatching tests?

Answer 22

demonstrate ABO incompatibility

demonstrate clinically significant Ab

Question 23

why is the washing procedure before the addition of AHG so important?

Answer 23

prevents netrualization of AHG by globulins in the blood

Question 24

FFP of the AB group is compatiable with what blood group recipients?

Answer 24

all blood groups. AB plasma does not have Abs