Presentation Terms Flashcards
What is HPLC used for
Separate, identify, and quantify each component in a mix
How is HPLC performed
Pump high pressure solvent (mobile phase) + sample mix into column with solid absorbent material
Sample separated by how it interacts with sorbent
Advantage of HPLC over normal LC
HPLC has smaller sorbent particle size so resolving power is greater
HPLC
High performance liquid chromatography
UV/Vis
Peak height is proportional to [ ] of the analyte
Absorbance is the fraction of radiation absorbed over a range of frequencies
deuterostome
Animals where the anus develops first, mouth later
protostome
mouth develops first then the anus
mammalian substrates insect AVE can hydrolyze
angiotensin I
substance P
luteinizing hormone releasing hormone
enkaphalins
enkephalinamides
angiotensin I
made from renin cleaving angiotensinogen
hydrolyzed by ACE removing two C-terminal residues to form angiotensin II
substance P
sensory neuropeptide involved in pain reception and inflammation
luteinizing hormone releasing hormone
degraded by ACE
normally causes release of FSH, LH – ovulation
enkephalins
peptides that regulate pain reception
enkephalinamides
mimic the effects of opiates
two types of mammalian ACE
somatic – 2 active domains found most importantly in the lung
testicular – one active site expressed in sperm
2 main functions of ACE
convert angiotensin I to angiotensin II
breakdown bradykinin a vasodilator and other vasoactive peptides
