Presentation 1: Analysis of expression profiles of long non coding Flashcards
What was the proposed hypothesis of this paper?
RABV infection will result in the expression of a unique profile of lncRNAs
What were the objectives of this paper?
1) To analyze the lncRNA expression profile in the brains of mice infected with RABV by RNA-sequencing
2) To verify the expression of select lncRNAs by RT-qPCR
What was the first result of this paper?
Sequence for total RNA
Found 944 novel lncRNAs
140 lncRNAs were differentially expressed at dpi 8
38 were upregulated
102 were downregulated
Most were seen in chromosome 2, 7, 16
Least were seen in chromosome 18, 19, and x
What was the second result of this paper?
Hierchal cluster analysis of mRNA expression profile, which was a RABV infected mice vs mock infected mice
3,807 mRNAs were differentially expressed in CVS-11 infected mice
2,187 were up regulated, 1620 were down regulated
Most: chromosome 2,7,11
Least: chromosome 16 and 18
None: Y chromosome
What did this paper state when comparing the genomic features of lncRNAs and mRNAs?
Lower expression of lncRNA
Lower exon number of lncRNA
Shorter transcript length of lncRNA
Shorter ORF of lncRNA
What is the biological significance of differential expression in lncRNAs?
140 lncRNAs were unregulated with a fold change of more than 30
KEGG pathway analysis showed target genes of these lncRNAs were unregulated in pathways like the T cell, Toll-like receptor, and signalling pathways
What is the taxonomy for the Rabies virus?
Family: Rhabdoviridae, Genus: Lyssavirus
What does the Rabies virus encode?
Nucleoprotein Phosphoprotein Matrix protein Glycoprotein RNA-dependent RNA polymerase
What is the genome of Rabies?
12 kb negative stranded RNA virus
Bullet shaped virion
What was the significance of this paper?
No curable treatment
Current vaccine needs multiple boosters and is expensive
Need to be able to clear RABV from CNS
Not yet known how RABV causes fatal disease
What were the methods employed in this study?
RNA sequencing and RT-qPCR
Explain the RABV infection cycle
Infection introduced from a bite
Surface glycoproteins bind to acetylcholine receptors on muscle or neuronal cells
RABV can remain at the site of introduction for up to 2 years
Virus will spread to the nervous system via neuromuscular junctions
How does the host respond to RABV?
In the periphery: Detected by pattern recognition receptors that activate nuclear factor B and interferon regulatory transcription factors
Type I INF response
In the CNS, recognized by RIG-I: NF-B and Type I IFN response
