Preparing And Observing A Cell In Mitosis Flashcards
What is the equation for mitotic index?
Mitotic index = number of cells undergoing mitosis / total number of cells
What type of cell must you cut from the plant to observe mitosis?
One from the tip a - meristematic cell
Describe the preparation of a slide to observe mitosis
Fixing - kill the cells with ethanoic alcohol and hydrochloric acid, stops cell division
Staining - place onto a slide and stain e.g. Toluidine blue
Squash the tip to produce a single cell layer, allowing light to pass through hence making the cells observable
Describe how to do a scientific drawing
No sketches - solid lines
No shading
No unbroken lines
How do you calculate the amount of time a cell spends in each stage of mitosis?
(Number of cells in the stage / total cells) x Total time
Describe how you use an eye piece graticule
Place a stage micrometer on microscope stage
Focus on scale
Align scale on eye piece graticule and stage micrometer
Count divisions on graticule equivalent to divisions on micrometer
Give the magnification equation
Magnification = image/actual
