PRELIM LEC: Leukocyte Development, Kinetics, and Functions Flashcards
How many leukocytes can be ID if someone will use flow cytometry?
5
CFU-L is also known as?
COLONY FORMING UNIT-LEUKOCYTE
CFU-GM will be acted upon by what? In order to become the first progenitor cell to become committed neutrophil
CFU-G
What is the first progenitor cell to become committed neutrophil?
CFU-G
It is a light stained part seen in cells e.g Promyelocyte.
Hof
what CD markers differentiate AML to APL?
AML cd34
APL cd64
Last Precursor cell to perform mitosis
Myelocyte
Starts the Dawn of neutrophilia
Myelocyte
. Adhesive molecules used for extravasation of neutrophils
Integrins and Selectins
Transit time of myoblast to myelocyte
4-6 days
Primary ROS of PMS
Hydrogen peroxide
What enzyme is used to convert the primary ROS to secondary ROS?
myeloperoxidase
. These are extracellular thread-like structures believed to represent chains of nucleosomes from unfolded nuclear chromatin material.
NET
. What are the 3 transcription factors used in the differentiation of eosinophil
GATA-1, C/EBP, and PU.1
Cytokines involved in the differentiation of basophils
IL-3, TSLP
. What interleukin is responsible for the long half-life of basophil?
IL-3, IL 25
The major cytokine responsible for MAST cell maturation and differentiation
Kit ligand
Beginning of the storage pool of neutrophils
METAMYELOCYTE (?)
Precursor that has a D-shaped Hof
MYELOCYTE
. Secondary ROS of PMNs
HYDROGEN CHLORITE
Factors needed for the production of Major Basic Protein
IL-3 AND IL-5
. Primary granule of eosinophil
CHARCOT LEYDEN CRYSTAL PROTEIN
. What enzyme is used for killing parasite?
PAPAIN
Give at least 3 ways of eosinophil degranulation
CLASSICAL EXOCYTOSIS
COMPOUND EXOCYTOSIS
PIECEMEAL DEGRANULATION
