prelim Flashcards
what is the DNA structure?
made up of two strands twisted to form a double helix
how are the two strands of DNA held together?
weak hydrogen bonds between the bases
what are the base pairings?
Adenine-Thymine , Cytosine-Guanine
what is DNA made up of?
subunits called nucleotides
what is a nucleotide made up of?
3 parts; A base, A phosphate and a deoxyribose sugar
what is PCR?
it is a way of making many copies of target regions of DNA
what is stage 1 of PCR?
DNA is heated to between 92° and 98° to separate the two strands (DNA is denatured)
what is stage two of PCR?
DNA is cooled to between 50 and 65°C to allow primers to bind (anneal) to target sequences
what is stage 3 of PCR?
DNA is heated to between 70 and 80°C for heat-tolerant DNA polymerase to replicate the region of DNA
what is transcription?
transcription is the name of the process in which a copy of DNA is made
what is the copy of DNA made from transcription called?
messenger RNA (mRNA)
where does transcription occur?
the nucleus
what is translation?
mRNA which has been transcribed is translated into proteins by ribosomes in the cytoplasm
what is a triplet of bases on mRNA called?
a codon
what is a non coding region of DNA called?
an intron