Pregnancy diagnosis Flashcards
Why cant you do cervical insemination with a super-ovulated ewe?
Too much mucous, sperm gets washed out by mucous
Lots of FSH - Lots of estrogen - lots of mucous
Why do embryo transfer?
Disseminate valuable female bloodlines
Get more offspring out of valuable females
Synchronisation & timing of progesterone sponge in superovulation
Progesterone sponge in for 12 days
48 hours prior to removal of sponge a combined shot of FSH and PMSG is given
FSH injections will be continued
Laproscopic insemination should be done 36 - 48 hours after sponge removal
Donar ewe should be injected with prostaglandin a week to ensure any eggs missed do not survive
Recipient ewes a prepared in the normal way and sponges are removed a day before donar ewes to ensure accurate timing as highly stimulated donars come into oestrous faster
- recipient ewes are given a dose of PMSG at time of sponge removal
How many recipient ewes should be prepared for insemination of donor oocytes?
5-10 per donar ewe
When are embryos typically flushed from donar ewes?
Day 6
Embryo flushing
Mid-ventral laporotomy under general anaesthesia
Tilted operating table
Incision of about 10 cm is made
Retrograde flush or folidcatheder
Catheder inserted via the infindibulum
How soon can donar ewews be bred naturally after oocyte flushing?
2 oestrous cycles
Implantation of embryos into recipient ewe
Modified laproscopic procedure
Local anaesthesia and sedation
The embryo will not survive unless it is placed ipsolaterally to the uterine horn with an active corpus luteum (closer to progesterone source and maternal recognition of pregnancy is closer to CL)
This method can only be used for embryos at the morulla or blastocyst stage
Average % of lambs from embryo transfer
60%
with around 4 to 5 lambs produced per donor treatment
