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Blood Bank > Pre-test > Flashcards

Pre-test Flashcards

1
Q

What are the reagents used in an antibody screen?

A

screening cells, LISS, and check cells

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2
Q

What type of patient specimen is used in an antibody screen?

A

patient serum or plasma

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3
Q

What is the purpose of the DAT?

A

Useful in detecting HDN, HTR, and AIHA

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4
Q

What are the components of a DAT?

A

AHG and patient cells

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5
Q

If an Rh control tube is necessary in the Rh testing, and it is positive, the interpretation of the Rh type is _______.

A

invalid

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6
Q

What are the two types of AHG that can be used in blood banking?

A

Broad spectrum/polyspecific and monospecific

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7
Q

Describe broad spectrum/polyspecific.

A

contains both anti-complement (C3d) and anti-IgG

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8
Q

Describe monospecific.

A

contains either anti-complement (C3d) or anti IgG

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9
Q

What is the purpose of the IAT?

A

detects antibodies or complement attached to RBCs in vitro

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10
Q

What are the components of the IAT?

A

patient serum and screening cells

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11
Q

What are two blood bank procedures that incorporate the IAT?

A

compatibility testing for donor cells and antibody screen

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12
Q

What are two tubes included in Du testing?

A

D test and Rh control

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13
Q

What are the steps for Du testing?

A
  1. incubate tubes at 37C for 15 min
  2. Using saline, wash cells 3 times
  3. Mix 2 drops AHG to each tube, centrifuge on high for 15 seconds
  4. Add 1 drop check cells to non-reactive tubes
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14
Q

In the fetal bleed screening test, the presence of agglutinated cells indicates that the maternal blood contains possibly a significant number of________.

A

Rh positive fetal cells

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15
Q

What term is used to describe the agglutination that constitutes a positive fetal bleed screening test?

A

rosette

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16
Q

What is the quantitative test performed when a fetal bleed screening test is positive?

A

Kleihauer-Betke test

17
Q

Who does the AABB require have the fetal bleed screening test performed?

A

Rh = mothers who deliver an Rh + baby

18
Q

What is another situation that may require a fetal bleed screening test?

A

abortion

19
Q

What are four conditions that the DAT can detect?

A

HDN, HTR, AIHA, and drug induced RBC sensitization

20
Q

What are the two components of an auto control?

A

patient red cells and patient serum

21
Q

What are the components of a crossmatch?

A

recipient plasma and donor RBCs

22
Q

Two cell populations in a patient specimen can produce what type of agglutination?

A

mixed-field agglutination

23
Q

What are the components of a forward ABO grouping?

A

anti-A and anti-B antisera and patient cells

24
Q

What are the components of a reverse ABO grouping?

A

patient serum and A1 and B cells

25
Q

What can be done to enhance an ABO reaction that is very weak upon immediate spin?

A

sit at RT for 30 min or incubate at 4C

26
Q

What blood bank procedure is used to resolve a positive DAT?

A

elution

27
Q

What blood bank procedure is used to resolve a positive antibody screen?

A

perform antibody panel to identify antibodies present

28
Q

Describe clinically significant antibodies.

A

IgG which react at 37C and in the AHG phase

29
Q

In a pregnant female, what antibody class is clinically significant?

A

Rh

30
Q

What is the Rh antibody class the most clinically significant in a pregnant female?

A

if a mother is Rh+ and baby is Rh= HDN could occur

31
Q

What are the two AABB requirements for routine crossmatching tests?

A

demonstrate ABO compatibility and clinically significant antibodies

32
Q

Why is the washing procedure before the addition of AHG so important?

A

to prevent neutralization of AHG by globulins present in blood

33
Q

FFP of the AB group is compatible with what blood group recipients?

A

all blood groups

34
Q

If check cells are added to a negative AHG tube, and after centrifugation agglutination is not seen, give two possible explanations.

A

inactive AHG or neutralized AHG

35
Q

What must be done if agglutination is not seen after addition of check cells to a negative AHG tube?

A

panel must be repeated

Blood Bank (1 decks)

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