pratical 12 seperation of species via thin layer chromotography Flashcards
1
Q
Outline the steps needed to locate the positions of the amino acids on the TLC plate and to determine their Rf values.
A
Spray with developing agent or use UV
Measure distances from initial pencil line to the spots (x)
Measure distance from initial pencil line to solvent front line (y)
Rf value = x / y
2
Q
why can the developing solvent not be at a depth more then 1cm
A
if the solvent is too deep it will dissolve the mixture from the plate
3
Q
why must we were gloves when carrying out thin layer chromotography
A
to prevent contamination of plate
4
Q
why is it not essential for the solvent to rise up the plate to the top
A
the Rf value can be calculated if the solvent front does not reach the top of the plate
