Practicum Flashcards
Antibodies that may cause a HDN
ABO, Rh, Kell, Duffy
Antibodies that may cause delayed HTR
Kidd
If R2R2 patient has a 3+ reaction and is compatible with R1r donor what Ab is present?
anti- E would be present
How are cold agglutinates and rouleaux excluded from testing?
Cold agglut- absorption
Rouleaux- saline replacement
List the antibodies detected at different phases.
AHG- Kell, Duffy, Kidd
IS- Lewis, MN,P1
37- Rh, MN
Interval between donations
8 weeks
minimum Hgb
minimum Hgb greater than or equal to 12.5 g/dL
minimum Hct
greater than or equal to 38%
Maximum total volume of blood drawn
525 mL max (normally 450 mL)
Minimum Body Weight
greater than or equal to 110 lbs (50kg)
Maximum Body Temperature
37.5 C/ 99.5 F
How long is the deferred period if person spent time in a malaria area?
1 year
Mother- R1r
child1- rr
child2- R2r
What would the father be?
R2r
What blood type is used for emergency release and alternate blood selection?
O negative blood
Refrigerator temperature
1-6 C
Length of time recipient samples are saved
7 days
Shelf life after breakage of hermetic seal
24 hours
Temperature range during whole blood shipment
1-10 C
Reason to discard a unit of blood
unit looks hemolyzed
Which blood group has the most reactivity with anti-H?
Group O is the strongest
Hemophiliac patient would need what type of blood component?
Cryoprecipitate
What is unique about the Lewis system?
The only blood group system not manufactured by the RBCs. Pregnant women may present as Le(a-b-) and produce Lewis antibody even though true phenotype is Le(a-b+). Can show up in saliva and tears.
What antibodies are found in Bombay individuals?
All of them (Anti-A, -B, -AB, -H)
What blood group antigens are enhanced or destroyed by enzymes?
Enhanced: Lewis, Rh, Kidd
Destroyed: Fy(a), Fy(b) (»Duffy Inhibited) MNS
In the panel given remember to choose the answer with…
2 negative and 1 positive for both antibodies
What would you expect if a Rh neg patient who has never been transfused or pregnant was transfused with Rh pos donor blood?
No transfusion reaction because no antibodies are present because no prior exposure
When should RhoGAM be given?
Rh negative mother with Rh positive baby
Elution
To determine allo or auto ab,
To determine HDN
Used to remove antibodies bound to sensitized RBCs
Forward grouping
Using forward known serum antibodies to detect unknown antigens
DAT
if POSITIVE perform elution to remove antibody to ID specific eluate
used to diagnose HDN, HTR & AIHA
also, in ((( Direct Coombs use patient cells!!!))))
Polyspecific AHG
Has both anti-complement/ IgG
What is Anti- A1 lectin used to detect?
used with A1- ABO discrepancy
Wharton’s jelly
on cord blood
Why aren’t reverse blood groupings done on newborns?
Antibodies are not yet developed
Define febrile nonhemolytic reaction
chills, fever, increase in temperature by 2 C
What could a weak subgroup of A cause?
ABO discrepancy
What would you use to distinguish between an A1/A2 discrepancy?
Dolichus Biflorus
If the mother is O and the baby is A and there is HDN what would you transfuse with?
Group O Blood
Would A2B with anti- A1 cause a discrepancy?
YES
What would the next step be to identify the cause of a strong positive DAT in cord blood and mother sample?
preform an elution on the sample
Why are the cells washed in the AHG test?
to prevent neutralization by globulins
What is the reason for a mixed- field result on a DAT for a patient who has been previously transfused?
2 cell populations in the patients blood
What type of sample do you need for compatibility testing?
FRESH serum
Why do you need a FRESH serum specimen?
To preserve complement
What type of elution is required for HDN
Acid Elution
Deferral
If they tested positive for HBsAg
