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MLT 280 Final St > Practicum > Flashcards

Practicum Flashcards

1
Q

Antibodies that may cause a HDN

A

ABO, Rh, Kell, Duffy

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2
Q

Antibodies that may cause delayed HTR

A

Kidd

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3
Q

If R2R2 patient has a 3+ reaction and is compatible with R1r donor what Ab is present?

A

anti- E would be present

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4
Q

How are cold agglutinates and rouleaux excluded from testing?

A

Cold agglut- absorption

Rouleaux- saline replacement

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5
Q

List the antibodies detected at different phases.

A

AHG- Kell, Duffy, Kidd
IS- Lewis, MN,P1
37- Rh, MN

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6
Q

Interval between donations

A

8 weeks

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7
Q

minimum Hgb

A

minimum Hgb greater than or equal to 12.5 g/dL

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8
Q

minimum Hct

A

greater than or equal to 38%

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9
Q

Maximum total volume of blood drawn

A

525 mL max (normally 450 mL)

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10
Q

Minimum Body Weight

A

greater than or equal to 110 lbs (50kg)

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11
Q

Maximum Body Temperature

A

37.5 C/ 99.5 F

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12
Q

How long is the deferred period if person spent time in a malaria area?

A

1 year

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13
Q

Mother- R1r
child1- rr
child2- R2r
What would the father be?

A

R2r

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14
Q

What blood type is used for emergency release and alternate blood selection?

A

O negative blood

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15
Q

Refrigerator temperature

A

1-6 C

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16
Q

Length of time recipient samples are saved

A

7 days

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17
Q

Shelf life after breakage of hermetic seal

A

24 hours

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18
Q

Temperature range during whole blood shipment

A

1-10 C

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19
Q

Reason to discard a unit of blood

A

unit looks hemolyzed

20
Q

Which blood group has the most reactivity with anti-H?

A

Group O is the strongest

21
Q

Hemophiliac patient would need what type of blood component?

A

Cryoprecipitate

22
Q

What is unique about the Lewis system?

A

The only blood group system not manufactured by the RBCs. Pregnant women may present as Le(a-b-) and produce Lewis antibody even though true phenotype is Le(a-b+). Can show up in saliva and tears.

23
Q

What antibodies are found in Bombay individuals?

A

All of them (Anti-A, -B, -AB, -H)

24
Q

What blood group antigens are enhanced or destroyed by enzymes?

A

Enhanced: Lewis, Rh, Kidd
Destroyed: Fy(a), Fy(b) (»Duffy Inhibited) MNS

25
Q

In the panel given remember to choose the answer with…

A

2 negative and 1 positive for both antibodies

26
Q

What would you expect if a Rh neg patient who has never been transfused or pregnant was transfused with Rh pos donor blood?

A

No transfusion reaction because no antibodies are present because no prior exposure

27
Q

When should RhoGAM be given?

A

Rh negative mother with Rh positive baby

28
Q

Elution

A

To determine allo or auto ab,
To determine HDN
Used to remove antibodies bound to sensitized RBCs

29
Q

Forward grouping

A

Using forward known serum antibodies to detect unknown antigens

30
Q

DAT

A

if POSITIVE perform elution to remove antibody to ID specific eluate
used to diagnose HDN, HTR & AIHA
also, in ((( Direct Coombs use patient cells!!!))))

31
Q

Polyspecific AHG

A

Has both anti-complement/ IgG

32
Q

What is Anti- A1 lectin used to detect?

A

used with A1- ABO discrepancy

33
Q

Wharton’s jelly

A

on cord blood

34
Q

Why aren’t reverse blood groupings done on newborns?

A

Antibodies are not yet developed

35
Q

Define febrile nonhemolytic reaction

A

chills, fever, increase in temperature by 2 C

36
Q

What could a weak subgroup of A cause?

A

ABO discrepancy

37
Q

What would you use to distinguish between an A1/A2 discrepancy?

A

Dolichus Biflorus

38
Q

If the mother is O and the baby is A and there is HDN what would you transfuse with?

A

Group O Blood

39
Q

Would A2B with anti- A1 cause a discrepancy?

A

YES

40
Q

What would the next step be to identify the cause of a strong positive DAT in cord blood and mother sample?

A

preform an elution on the sample

41
Q

Why are the cells washed in the AHG test?

A

to prevent neutralization by globulins

42
Q

What is the reason for a mixed- field result on a DAT for a patient who has been previously transfused?

A

2 cell populations in the patients blood

43
Q

What type of sample do you need for compatibility testing?

A

FRESH serum

44
Q

Why do you need a FRESH serum specimen?

A

To preserve complement

45
Q

What type of elution is required for HDN

A

Acid Elution

46
Q

Deferral

A

If they tested positive for HBsAg

MLT 280 Final St (2 decks)

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